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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Screening of organic supports and activation protocols for immobilization and stabilization of lipase from Thermomyces lanuginosus

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Author(s):
Adriano A. Mendes [1] ; Heizir F. de Castro [2] ; Raquel de L. C. Giordano [3]
Total Authors: 3
Affiliation:
[1] Universidade Federal de São João Del-Rei - Brasil
[2] Universidade de São Paulo. Escola de Engenharia de Lorena. Departamento de Engenharia Química - Brasil
[3] Universidade Federal de São Carlos. Departamento de Engenharia Química - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Química Nova; v. 36, n. 2, p. 245-251, 2013-00-00.
Abstract

Lipase from Thermomyces lanuginosus was covalently immobilized on activated poly-hydroxybutyrate, sugarcane bagasse and the chemically modified hybrid hydrogel chitosan-alginate prepared by different strategies. Among the tested supports, chitosan-alginate chemically modified with 2,4,6-trinitrobenzenesulfonic acid rendered derivatives with the highest hydrolytic activity and thermal-stability, 45-fold more stable than soluble lipase and was then selected for further studies. The pH of maximum activity was similar for both immobilized and free lipase (pH 8.0) while optimum temperature was 5 - 10 ºC higher for the immobilized lipase. Higher yields in the butyl butyrate synthesis were found for the derivatives prepared by activation with glycidol and epichlorohydrin. (AU)