| Full text | |
| Author(s): |
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01255 Sao Paulo - Brazil
[2] Oxitec Ltd, Oxford - England
[3] Univ Oxford, Dept Zool, Oxford OX1 3PS - England
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Memórias do Instituto Oswaldo Cruz; v. 108, n. 4, p. 529-531, 2013-06-00. |
| Abstract | |
Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues. We were able to express DsRed2 fluorescent protein in adult Cx. quinquefasciatus mosquitoes by injecting plasmids directly into their thorax. The expression of DsRed2 in adult Cx. quinquefasciatus mosquitoes is an important stepping stone to genetic transformation and the potential use of new control strategies and genetic interactions. (AU) | |
| FAPESP's process: | 05/50225-2 - Development of alternative methodologies in the control of mosquitoes (Diptera: culicidae) of epidemiological importance: use of method RIDL (release of insects carrying a dominant lethal gene) in the control of Culex quinquefasciatus mosquitoes |
| Grantee: | Mauro Toledo Marrelli |
| Support Opportunities: | Research Grants - Young Investigators Grants |