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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Etiology and pathogenicity of two different isolates of Colletotrichum spp. obtained from physic nut seeds

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Author(s):
Gil Rodrigues dos Santos [1] ; Hugo José Tozze Júnior [2] ; Danila Alves Corrêa de Sá [3] ; Gleiber Quintão Furtado [4] ; Nelson Sidnei Massola Júnior [5]
Total Authors: 5
Affiliation:
[1] Universidade Federal do Tocantins. Departamento de Fitopatologia - Brasil
[2] Universidade de São Paulo. Departamento de Fitopatologia e Nematologia - Brasil
[3] Universidade Federal do Tocantins. Departamento de Fitopatologia - Brasil
[4] Universidade Federal de Viçosa,. Departamento de Fitopatologia - Brasil
[5] Universidade de São Paulo. Departamento de Fitopatologia e Nematologia - Brasil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF SEED SCIENCE; v. 35, n. 2, p. 139-146, 2013-00-00.
Abstract

The species known as physic nut (Jatropha curcas L.) has become important as one of main sources of feedstock for biodiesel production. The aims of this study were characterizing two different isolates of Colletotrichum spp. obtained from seeds of this species, through morphological, cultural, and molecular analyses; as well as assessing pathogenicity of both isolates on leaves and fruit of this plant species. For morphological analysis, length and width of 30 spores of each isolate, produced on medium Malt Extract-Agar (MEA), at 25 ± 1 ºC, and under constant light, were measured. Cultural analysis was performed by the fungus growth on PDA medium, through daily measurements of the mycelial growth, and the color of colonies after seven days incubation. DNA of the isolates was extracted and specific oligonucleotides primers (region ITS4) were identified by PCR and used to identify C. capsici (CcInt) and C. gloeosporioides (CgInt). Pathogenicity of isolates was assessed on plants aged 10, 20, 30, 40, 50, and 60 days after sowing and fruits at six maturation stages. Based on these analyses, isolates were identified as C. gloeosporioides and C. capsici. Young plants and the older fruits were more susceptible to infection by the two fungus species. (AU)

FAPESP's process: 11/11629-1 - Brazilian anthracnosis: identification, characterization and variability of causal agents
Grantee:Nelson Sidnei Massola Júnior
Support Opportunities: Regular Research Grants