Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions

Author(s)

C. Bellão ^[1] ; T. Antonio ^[2] ; M. L. G. C. Araujo ^[2] ; A. C. Badino ^[3]

Affiliation

^[1] Universidade Federal de São Carlos. Department of Chemical Engineering - Brasil

^[2] Univ Estadual Paulista, Inst Chem, Dept Biochem & Technol, Araraquara, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Chemical Engineering; v. 30, n. 2, p. 257-266, 2013-06-00.

Abstract

The effect of carbon source and feeding conditions on the production of clavulanic acid (CA) and cephamycin C (CephC) by Streptomyces clavuligerus was investigated. In fed-batch experiments performed with glycerol feeding, production of CA exceeded that of CephC, and reached 1022 mg.L-1. Highest CephC production (566.5 mg.L-1) was obtained in fed-batch cultivation with glycerol feeding. In fed-batch experiments performed with starch feeding, the production of CephC was in general higher than that of CA. A dissociation index (DI) was used to identify feeding conditions that favored production of CephC relative to CA. In all cultures with glycerol, DI values were less than unity, indicating higher production of CA compared to CephC. Conversely, in cultures fed with starch, the DI values obtained were greater than unity. However, no carbon source or feeding condition was able to completely dissociate the production of CA from that of CephC. (AU)

• FAPESP's process: 11/23807-1 - Development and evaluation of the operational conditions of bioreactors for production of biotechnological products
• Grantee: Alberto Colli Badino Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces
• Grantee: Carlos Osamu Hokka
• Support Opportunities: Research Projects - Thematic Grants