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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Temporal and Behavioral Variability in Cannabinoid Receptor Expression in Outbred Mice Submitted to Ethanol-Induced Locomotor Sensitization Paradigm

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Coelhoso, Cassia C. [1] ; Engelke, Douglas S. [1] ; Filev, Renato [1] ; Silveira, Dartiu X. [2] ; Mello, Luiz E. [1] ; Santos-Junior, Jair G. [1, 3]
Total Authors: 6
[1] Univ Fed Sao Paulo, Neurobiol Lab, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Psychiat & Med Psychol, Sao Paulo - Brazil
[3] Fac Ciencias Med Santa Casa, Dept Physiol Sci, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH; v. 37, n. 9, p. 1516-1526, SEP 2013.
Web of Science Citations: 12

Background There is a close relationship between the endocannabinoid system and alcoholism. This study investigated possible differential expression of cannabinoid receptors CB1 (CB1R) and CB2 (CB2R) in an outbred mice strain displaying behavioral variability to ethanol (EtOH)-induced locomotor sensitization. Methods Male adult Swiss mice treated chronically with EtOH (2g/kg, i.p., daily for 21days) were classified as EtOH\_High or EtOH\_Low according to their locomotor activity after the 21st EtOH injection. A control group was similarly injected with saline. Temporal analysis of CB1R and CB2R immunoreactivity was performed in 3 different occasions: (i) at the end of chronic EtOH treatment, (ii) on the fifth day of EtOH withdrawal, and (iii) after EtOH challenge. Results Overall, no differences were seen between experimental groups regarding the CB1R at the end of acquisition. However, there were decreases in CB2R in the prefrontal cortex and the hippocampus in EtOH\_Low mice. On the fifth day of withdrawal, only EtOH\_High mice presented increase in CB1R. Nonetheless, CB2R up-regulation was observed in both EtOH\_High and EtOH\_Low mice. EtOH challenge counteracted CB1R and CBR2 up-regulation, mainly in the EtOH\_High, in structures related to emotionality, such as prefrontal cortex, ventral tegmental area, amygdala, striatum, and hippocampus. Conclusions There are different patterns of cannabinoid receptor expression during locomotor sensitization paradigm, at both temporal and behavioral perspectives. We hypothesize that CB2R down-regulation might be related to resilience to develop locomotor sensitization, while CB1R up-regulation relates to withdrawal aspects in sensitized mice. (AU)

FAPESP's process: 11/03184-0 - Epigenetic mechanisms between pCREB AND GLUR2 in the locomotor sensitization induced by ethanol in mice
Grantee:Cássia de Canha Coelhoso
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/16305-0 - PKM participation on the contextual influence in locomotor sensitization induced by anfetamine
Grantee:Douglas Senna Engelke
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 09/16307-2 - Behavioral, neurochemical and molecular effects of cannabidiol in mice submitted to locomotor sensitization induced by ethanol
Grantee:Renato Filev
Support type: Scholarships in Brazil - Doctorate (Direct)