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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Humoral and Cell-Mediated Immune Responses to Different Doses of Attenuated Vaccine Against Avian Infectious Bronchitis Virus

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Author(s):
Okino, Cintia Hiromi [1, 2] ; Alessi, Antonio Carlos [1] ; Silva Montassier, Maria de Fatima [1] ; de Magalhaes Rosa, Artur Jordao [3] ; Wang, Xiuqing [4] ; Montassier, Helio Jose [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Dept Vet Pathol, Lab Immunol & Virol, BR-14884900 Jaboticabal, SP - Brazil
[2] Embrapa Swine & Poultry, Concordia, SC - Brazil
[3] Embrapa Cerrados, Brasilia, DF - Brazil
[4] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 - USA
Total Affiliations: 4
Document type: Journal article
Source: VIRAL IMMUNOLOGY; v. 26, n. 4, p. 259-267, AUG 2013.
Web of Science Citations: 19
Abstract

The antibody and cellular immune responses against infectious bronchitis virus (IBV) were evaluated at mucosal sites of chickens after immunization with various doses of an attenuated vaccine at 1 day of age. The correlation of these immune responses with protection of tracheal tissues was evaluated after experimental infection of these birds. Significantly reduced tracheal pathologic effects, measured according to ciliostasis and histology lesions, and reduced viral load were observed only in the full-dose vaccinated group at 5 days post-infection (dpi), while incomplete protection was observed for the subdose vaccinated groups. Moreover, birds of vaccinated groups, especially with full dose, developed higher levels of lachrymal IBV-specific IgG and IgA and increased the expression of cell-mediated immunity (CMI) genes, such as gamma interferon (IFN gamma), CD8+ T cell marker, and granzyme homolog A more rapidly. In addition, these humoral and cellular immune responses evaluated at mucosal sites correlated significantly with tracheal protection against homologous IBV challenge in a vaccine dose-dependent manner. The results indicate that IgG, IgA and CD8+ T cell responses developed at mucosal sites after IBV vaccination of day-old chicks, could be taken as good correlates of protection against this virus. (AU)