The anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in mice

Author(s)

de Morais-Zani, Karen ^{[1, 2]} ; Barbosa Nunes, Fernanda Peixoto ^[3] ; da Silva, Jacilene Barbosa ^[1] ; Ferreira, Marcio Jose ^[4] ; Grego, Kathleen Fernandes ^[1] ; Lopes-Ferreira, Monica ^[4] ; Tanaka, Aparecida Sadae ^[5] ; Tanaka-Azevedo, Anita Mitico ^{[1, 2]}

Affiliation

^[1] Inst Butantan, Lab Herpetol, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Programa Posgrad Interunidades Biotecnol, Inst Pesquisas Tecnol, Inst Butantan, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, BR-05508900 Sao Paulo - Brazil

^[4] Inst Butantan, CEPID FAPESP, Lab Especial Toxinolgia Aplicada, BR-05503900 Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo - Brazil

• Document type: Journal article
• Source: Inflammation Research; v. 62, n. 8, p. 733-742, AUG 2013.
• Web of Science Citations: 2

Abstract

Antithrombin is known as the most important natural coagulation inhibitor and has been shown to have anti-inflammatory properties. The present study aimed to investigate the effects of Bothrops jararaca antithrombin on acute inflammation induced by carrageenan in mice. We evaluated the anti-inflammatory activity of antithrombin on models of paw edema formation, cell migration and leukocyte-endothelium interaction in mice (Swiss; n = 5). Acute inflammation was induced by the administration of carrageenan (15 mg kg(-1)). Treatment with B. jararaca antithrombin (1 mg kg(-1)) 1 h before or after carrageenan administration significantly inhibited paw edema formation, reduced cell influx to the peritoneal cavity due to reduction in the migration of polymorphonuclear cells, and attenuated leukocyte rolling in the microcirculation of the cremaster muscle.The effects of antithrombin on vascular and cellular events of inflammation were completely abolished by treatment with the cyclo-oxygenase inhibitor indomethacin (4 mg kg(-1)), suggesting the involvement of prostacyclin in the mechanism of inflammation inhibition by B. jararaca antithrombin. This work showed for the first time the anti-inflammatory properties of B. jararaca antithrombin on vascular and cellular events of inflammation. These findings suggest that antithrombin is effective in preventing paw edema formation, cell migration and leukocyte rolling induced by carrageenan in mice. (AU)

• FAPESP's process: 11/21558-4 - Study of anti-inflammatory activity of Bothropoides jararaca antithrombin by proteomic analysis
• Grantee: Karen de Morais Zani
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 09/03484-3 - Study of anti-inflammatory activity of antithrombin of Bothrops jararaca snake: antithrombin cloning and expression
• Grantee: Anita Mitico Tanaka-Azevedo
• Support Opportunities: Regular Research Grants