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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rescue of Amyloid-Beta-Induced Inhibition of Nicotinic Acetylcholine Receptors by a Peptide Homologous to the Nicotine Binding Domain of the Alpha 7 Subtype

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Author(s):
Nery, Arthur A. [1] ; Magdesian, Margaret H. [2] ; Trujillo, Cleber A. [1] ; Sathler, Luciana B. [2] ; Juliano, Maria A. [3] ; Juliano, Luiz [3] ; Ulrich, Henning [1] ; Ferreira, Sergio T. [2]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[2] Univ Fed Rio de Janeiro, Inst Med Biochem, Rio De Janeiro, RJ - Brazil
[3] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 8, n. 7 JUL 22 2013.
Web of Science Citations: 7
Abstract

Alzheimer's disease (AD) is characterized by brain accumulation of the neurotoxic amyloid-beta peptide (A beta) and by loss of cholinergic neurons and nicotinic acetylcholine receptors (nAChRs). Recent evidence indicates that memory loss and cognitive decline in AD correlate better with the amount of soluble A beta than with the extent of amyloid plaque deposits in affected brains. Inhibition of nAChRs by soluble A beta 40 is suggested to contribute to early cholinergic dysfunction in AD. Using phage display screening, we have previously identified a heptapeptide, termed IQ, homologous to most nAChR subtypes, binding with nanomolar affinity to soluble A beta 40 and blocking A beta-induced inhibition of carbamylcholine-induced currents in PC12 cells expressing alpha 7 nAChRs. Using alanine scanning mutagenesis and whole-cell current recording, we have now defined the amino acids in IQ essential for reversal of A beta 40 inhibition of carbamylcholine-induced responses in PC12 cells, mediated by alpha 7 subtypes and other endogenously expressed nAChRs. We further investigated the effects of soluble A beta, IQ and analogues of IQ on alpha 3 beta 4 nAChRs recombinantly expressed in HEK293 cells. Results show that nanomolar concentrations of soluble A beta 40 potently inhibit the function of alpha 3 beta 4 nAChRs, and that subsequent addition of IQ or its analogues does not reverse this effect. However, co-application of IQ makes the inhibition of alpha 3 beta 4 nAChRs by A beta 40 reversible. These findings indicate that A beta 40 inhibits different subtypes of nAChRs by interacting with specific receptor domains homologous to the IQ peptide, suggesting that IQ may be a lead for novel drugs to block the inhibition of cholinergic function in AD. (AU)

FAPESP's process: 06/61285-9 - Molecular basis of differentiation of stem and neural progenitor cells
Grantee:Alexander Henning Ulrich
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications
Grantee:Alexander Henning Ulrich
Support Opportunities: Research Projects - Thematic Grants