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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic diversity of Ehrlichia canis in Brazil

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Author(s):
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Aguiar, D. M. [1] ; Zhang, X. [2, 3, 4] ; Melo, A. L. T. [1] ; Pacheco, T. A. [1] ; Meneses, A. M. C. [5] ; Zanutto, M. S. [6] ; Horta, M. C. [7] ; Santarem, V. A. [8] ; Camargo, L. M. A. [9] ; McBride, J. W. [2, 3, 4] ; Labruna, M. B. [10]
Total Authors: 11
Affiliation:
[1] Univ Fed Mato Grosso, Hosp Vet, Lab Virol & Rickettsioses, BR-78060900 Cuiaba, MT - Brazil
[2] Univ Texas Med Branch, Dept Pathol, Ctr Biodef & Emerging Infect Dis, Sealy Ctr Vaccine Dev, Galveston, TX 77555 - USA
[3] Univ Texas Med Branch, Inst Human Infect & Immun, Galveston, TX 77555 - USA
[4] Univ Texas Med Branch, Dept Microbiol & Immunol, Ctr Biodef & Emerging Infect Dis, Sealy Ctr Vaccine Dev, Galveston, TX 77555 - USA
[5] Univ Fed Rural Amazonia, Inst Saude & Prod Anim, Belem, Para - Brazil
[6] Univ Estadual Londrina, Ctr Ciencias Agr, Dept Clin Vet, Londrina, PR - Brazil
[7] Univ Fed Vale Sao Francisco, Colegiado Curso Med Vet, Petrolina, PE - Brazil
[8] Univ Oeste Paulista, Hosp Vet, Lab Med Vet Prevent 2, Presidente Prudence, SP - Brazil
[9] Univ Sao Paulo, Inst Ciencias Biomed 5, Monte Negro, RO - Brazil
[10] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo - Brazil
Total Affiliations: 10
Document type: Journal article
Source: Veterinary Microbiology; v. 164, n. 3-4, p. 315-321, JUN 28 2013.
Web of Science Citations: 22
Abstract

Canine monocytic ehrlichiosis is a highly prevalent disease in Brazil, where the genetic diversity of Ehrlichia canis remains undefined. In this study, we used the TRP36 gene to examine the genetic diversity of E. canis strains from naturally infected dogs residing in five distinct geographic regions in Brazil. E. canis DNA was detected in 82/126 (65%) dogs by dsb-specific PCR and E. canis was isolated in cell culture from 13 dogs. Sequences obtained from dsb genes amplified from the isolates were identical to the US E. canis strain. An extended molecular characterization based on the TRP36 gene identified two major genogroups based on differences among eight isolates. Isolates with tandem repeat amino acid sequence (TEDSVSAPA) identical to the previously reported TRP36 sequence were found in the midwest, northeast and southeast regions of Brazil, and classified into the US genogroup. A novel Brazilian genotype with a different tandem repeat sequence (ASVVPEAE) was also identified in midwest, northern and southern regions. Similarity in the N-terminal sequence of a US genogroup member with the Brazilian genogroup suggested that genomic recombination between the two genogroups may have occurred. Other subtypes within the Brazilian genogroup were also identified using C-terminal amino acid divergence. We identified two distinct major Brazilian genogroups and several subtypes based on analysis of TRP36, and such information will be useful for further genotyping and possible associations with disease severity, understanding of the genetic and antigenic variability of E. canis, and for developing strain-specific vaccines and diagnostic methods based on TRP36. (c) 2013 Elsevier B.V. All rights reserved. (AU)