| Full text | |
| Author(s): |
Total Authors: 3
|
| Affiliation: | [1] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Biol, Rio Claro, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | PLoS One; v. 8, n. 6 JUN 27 2013. |
| Web of Science Citations: | 55 |
| Abstract | |
Supernumerary chromosomes (B chromosomes) occur in approximately 15% of eukaryote species. Although these chromosomes have been extensively studied, knowledge concerning their specific molecular composition is lacking in most cases. The accumulation of repetitive DNAs is one remarkable characteristic of B chromosomes, and the occurrence of distinct types of multigene families, satellite DNAs and some transposable elements have been reported. Here, we describe the organization of repetitive DNAs in the A complement and B chromosome system in the grasshopper species Abracris flavolineata using classical cytogenetic techniques and FISH analysis using probes for five multigene families, telomeric repeats and repetitive C(0)t-1 DNA fractions. The 18S rRNA and H3 histone multigene families are highly variable and well distributed in A. flavolineata chromosomes, which contrasts with the conservation of U snRNA genes and less variable distribution of 5S rDNA sequences. The H3 histone gene was an extensively distributed with clusters occurring in all chromosomes. Repetitive DNAs were concentrated in C-positive regions, including the pericentromeric region and small chromosomal arms, with some occurrence in C-negative regions, but abundance was low in the B chromosome. Finally, the first demonstration of the U2 snRNA gene in B chromosomes in A. flavolineata may shed light on its possible origin. These results provide new information regarding chromosomal variability for repetitive DNAs in grasshoppers and the specific molecular composition of B chromosomes. (AU) | |
| FAPESP's process: | 11/19481-3 - Contributions to elucidation of B chromosome biological roles using as model Rhammatocerus brasiliensis (Orthoptera) and Haplochromis obliquidens (Teleostei): Structure, evolution and histone proteins modification patterns |
| Grantee: | Diogo Cavalcanti Cabral-de-Mello |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/18028-3 - Isolation, characterization and chromosomal mapping of repeated DNA sequences in Abracris flavolineata (Acrididae, Ommatolampinae), with enphasis in the B chromosome analysis |
| Grantee: | Danilo Bueno |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |