| Full text | |
| Author(s): |
Total Authors: 4
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| Affiliation: | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 2
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| Document type: | Journal article |
| Source: | Journal of Molecular Biology; v. 425, n. 12, p. 2174-2197, JUN 26 2013. |
| Web of Science Citations: | 24 |
| Abstract | |
Signal transduction pathways mediated by cyclic-bis(3' -> 5')-dimeric GMP (c-di-GMP) control many important and complex behaviors in bacteria. C-di-GMP is synthesized through the action of GGDEF domains that possess diguanylate cyclase activity and is degraded by EAL or HD-GYP domains with phosphodiesterase activity. There is mounting evidence that some important c-di-GMP-mediated pathways require protein-protein interactions between members of the GGDEF, EAL, HD-GYP and PilZ protein domain families. For example, interactions have been observed between PilZ and the EAL domain from FimX of Xanthomonas citri (Xac). FimX and PilZ are involved in the regulation of type IV pilus biogenesis via interactions of the latter with the hexameric PilB ATPase associated with the bacterial inner membrane. Here, we present the crystal structure of the ternary complex made up of PilZ, the FimX EAL domain (FimX(EAL)) and c-di-GMP. PilZ interacts principally with the lobe region and the N-terminal linker helix of the FimX(EAL). These interactions involve a hydrophobic surface made up of amino acids conserved in a non-canonical family of PilZ domains that lack intrinsic c-di-GMP binding ability and strand complementation that joins beta-sheets from both proteins. Interestingly, the c-di-GMP binds to isolated FimX(EAL) and to the PilZ-FimX(EAL) complex in a novel conformation encountered in c-di-GMP-protein complexes in which one of the two glycosidic bonds is in a rare syn conformation while the other adopts the more common anti conformation. The structure points to a means by which c-di-GMP and PilZ binding could be coupled to FimX and PilB conformational states. (c) 2013 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 11/07777-5 - Cyclic di-GMP signaling and the Type IV macromolecule secretion system in Xanthomonas citri |
| Grantee: | Shaker Chuck Farah |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 11/22571-4 - Regulation of Xanthomonas citri Type IV pilus |
| Grantee: | Ricardo Germán Dunger |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/14477-8 - Functional and structural studies of PilZXAC1133-FimXXAC2398 - PilBXAC3239 complex from Xanthomonas axonopodis pv citri |
| Grantee: | Cristiane Rodrigues Guzzo Carvalho |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |