| Full text | |
| Author(s): |
Furuya, D. T.
[1]
;
Neri, E. A.
[1]
;
Poletto, A. C.
[1]
;
Anhe, G. F.
[2]
;
Freitas, H. S.
[1]
;
Campello, R. S.
[1]
;
Reboucas, N. A.
[1]
;
Machado, U. F.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Molecular and Cellular Endocrinology; v. 370, n. 1-2, p. 87-95, MAY 6 2013. |
| Web of Science Citations: | 17 |
| Abstract | |
Glucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-kappa B transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative kappa B sites located inside -83/-62 and -134/-113 bp. Eletrophoretic mobility assay showed that p50 and p65 NF-kappa B subunits bind to both putative kappa B sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-kappa B binds to the -134/-113 bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. The current findings demonstrate the existence of two kappa B sites in Slc2a4 gene promote, and that NF-kappa B has a direct repressor effect upon the Slc2a4 gene, providing an important link between insulin resistance and inflammation. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 07/50554-1 - Glucose transporters and diabetes mellitus: contribution to the knowledge of glycemic control and chronic diseases development |
| Grantee: | Ubiratan Fabres Machado |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 11/08570-5 - Nuclear factor kappa B (NF-kB) induced regulation of SLC2A4 gene expression: direct transcriptional control? |
| Grantee: | Ubiratan Fabres Machado |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 08/09194-4 - Cannabinoid receptor CB1 modulation of glucose transporter protein GLUT4 in adipocytes 3T3-L1 and visceral adipose tissue of obese mice |
| Grantee: | Daniela Tomie Furuya |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |