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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of nuclear factor-kappa B sites in the Slc2a4 gene promoter

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Furuya, D. T. [1] ; Neri, E. A. [1] ; Poletto, A. C. [1] ; Anhe, G. F. [2] ; Freitas, H. S. [1] ; Campello, R. S. [1] ; Reboucas, N. A. [1] ; Machado, U. F. [1]
Total Authors: 8
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 370, n. 1-2, p. 87-95, MAY 6 2013.
Web of Science Citations: 17

Glucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-kappa B transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative kappa B sites located inside -83/-62 and -134/-113 bp. Eletrophoretic mobility assay showed that p50 and p65 NF-kappa B subunits bind to both putative kappa B sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-kappa B binds to the -134/-113 bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. The current findings demonstrate the existence of two kappa B sites in Slc2a4 gene promote, and that NF-kappa B has a direct repressor effect upon the Slc2a4 gene, providing an important link between insulin resistance and inflammation. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 07/50554-1 - Glucose transporters and diabetes mellitus: contribution to the knowledge of glycemic control and chronic diseases development
Grantee:Ubiratan Fabres Machado
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/09194-4 - Cannabinoid receptor CB1 modulation of glucose transporter protein GLUT4 in adipocytes 3T3-L1 and visceral adipose tissue of obese mice
Grantee:Daniela Tomie Furuya
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/08570-5 - Nuclear factor kappa B (NF-kB) induced regulation of SLC2A4 gene expression: direct transcriptional control?
Grantee:Ubiratan Fabres Machado
Support type: Regular Research Grants