Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of nuclear factor-kappa B sites in the Slc2a4 gene promoter

Full text
Author(s):
Furuya, D. T. [1] ; Neri, E. A. [1] ; Poletto, A. C. [1] ; Anhe, G. F. [2] ; Freitas, H. S. [1] ; Campello, R. S. [1] ; Reboucas, N. A. [1] ; Machado, U. F. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 370, n. 1-2, p. 87-95, MAY 6 2013.
Web of Science Citations: 17
Abstract

Glucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-kappa B transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative kappa B sites located inside -83/-62 and -134/-113 bp. Eletrophoretic mobility assay showed that p50 and p65 NF-kappa B subunits bind to both putative kappa B sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-kappa B binds to the -134/-113 bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. The current findings demonstrate the existence of two kappa B sites in Slc2a4 gene promote, and that NF-kappa B has a direct repressor effect upon the Slc2a4 gene, providing an important link between insulin resistance and inflammation. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

FAPESP's process: 07/50554-1 - Glucose transporters and diabetes mellitus: contribution to the knowledge of glycemic control and chronic diseases development
Grantee:Ubiratan Fabres Machado
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/09194-4 - Cannabinoid receptor CB1 modulation of glucose transporter protein GLUT4 in adipocytes 3T3-L1 and visceral adipose tissue of obese mice
Grantee:Daniela Tomie Furuya
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/08570-5 - Nuclear factor kappa B (NF-kB) induced regulation of SLC2A4 gene expression: direct transcriptional control?
Grantee:Ubiratan Fabres Machado
Support type: Regular Research Grants