Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

To what extent is sleep rebound effective in reversing the effects of paradoxical sleep deprivation on gene expression in the brain?

Full text
Author(s):
Guindalini, Camila [1] ; Andersen, Monica L. [1] ; Alvarenga, Tathiana [1] ; Lee, Kil [1] ; Tufik, Sergio [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Behavioural Brain Research; v. 201, n. 1, p. 53-58, July 2009.
Field of knowledge: Humanities - Psychology
Web of Science Citations: 22
Abstract

Sleep is essential to maintaining health and well-being. It has been demonstrated that some of the biological alterations caused by paradoxical sleep deprivation (PSD) are not completely reversed after a period of sleep rebound (SR). The purpose of this study was to determine to what extent the specific molecular changes that occur in the rat cerebral cortex after 96 h of PSD can effectively be reversed during 24 h of recovery. Total RNA from the right cerebral cortex of Wistar male rats and GeneChip® Rat Genome 230 2.0 arrays were used to perform comprehensive microarray analysis of gene expression in control, PSD and SR groups. Microarray data were validated by Real Time qPCR. A total of 78 unique transcripts were differently expressed after PSD relative to control levels. These include genes related to metabolic processes, the circadian sleep-wake cycle, response to stimuli, regulation of cell proliferation and signaling pathways. After 24 h of sleep rebound, 62% of the sleep deprivation transcripts were again detected as differently expressed in the SR relative to the PSD group, although in the opposite direction. On the other hand, the expression of the remaining transcripts showed intermediate values between control and sleep-deprived animals. In summary, our results provide a unique set of transcripts that might be specific related to regulation of paradoxical sleep phase and sleep homeostasis processes, as well as to the biological basis of sleep disorders. (AU)

FAPESP's process: 98/14303-3 - Center for Sleep Studies
Grantee:Sergio Tufik
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC