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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biological activities from extracts of endophytic fungi isolated from Viguiera arenaria and Tithonia diversifolia

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Author(s):
Guimarães, Denise O. ; Borges, Warley S. ; Kawano, Cristina Y. ; Ribeiro, Patrícia H. ; Goldman, Gustavo H. ; Nomizo, Auro ; Thiemann, Otávio H. ; Oliva, Glaucius ; Lopes, Norberto P. ; Pupo, Monica T. [10]
Total Authors: 10
Document type: Journal article
Source: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY; v. 52, n. 1, p. 134-144, 2008.
Field of knowledge: Physical Sciences and Mathematics - Chemistry
Abstract

A total of 39 endophytic fungi have been isolated from Viguiera arenaria and Tithonia diversifolia, both collected in Sao Paulo State, Brazil. The isolates were identified based on their ribosomal DNA sequences. The ethyl acetate (EtOAc) extracts of all endophytic fungi were evaluated for their antimicrobial, antiparasitic and antitumoral activity. Antimicrobial screening was conducted using an agar diffusion assay against three pathogenic microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. Antiparasitic activity was determined by enzymatic inhibition of gGAPDH of Trypanosoma cruzi and adenine phosphorybosiltransferase (APRT) of Leishmania tarentolae. Antitumoral activity was tested against human T leukemia cells by theMosmann colorimetric method. All extracts showed activity in at least one assay: 79.5% of the extracts were cytotoxic against leukemia cells, 5.1% of the extracts were active against S. aureus, 25.6% against E. coli and 64.1% against Candida albicans. Only one extract showed promising results in the inhibition of parasitic enzymes gGAPDH (95.0%) and three were found to inhibit APRT activity. The cytotoxic extract produced by the strain VA1 (Glomerella cingulata) was fractionated and yielded nectriapyrone and tyrosol. Nectriapyrone showed relevant cytotoxic activity against both human T leukemia and melanoma tumor cell lines. (AU)

FAPESP's process: 04/07935-6 - Bioprospection in fungi: the search of lead compounds for drug design and enzymes for pharmaceutical and industrial applications
Grantee:Jairo Kenupp Bastos
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants