Search for optimum conditions of sugarcane bagasse hemicellulose extraction

Author(s)

Brienzo, M. ^[1] ; Siqueira, A. F. ^[1] ; Milagres, A. M. F. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Engn Lorena, Dept Biotechnol, BR-12602810 Lorena, SP - Brazil

• Document type: Journal article
• Source: Biochemical Engineering Journal; v. 46, n. 2, p. 199-204, Oct. 2009.
• Field of knowledge: Biological Sciences - Microbiology
• Web of Science Citations: 80

Abstract

A process has been elaborated for one-step low lignin content sugarcane bagasse hemicellulose extraction using alkaline solution of hydrogen peroxide. To maximize the hemicellulose yields several extraction conditions were examined applying the 24 factorial design: H2O2 concentration from 2 to 6% (w/v), reaction time from 4 to 16 h, temperature from 20 to 60 °C, and magnesium sulfate absence or presence (0.5%, w/v). This approach allowed selection of conditions for the extraction of low and high lignin content hemicellulose. At midpoint the yield of hemicellulose was 94.5% with more than 88% of lignin removed. Lignin removal is suppressed at low extraction temperatures and in the absence of magnesium sulfate. Hemicellulose in 86% yield with low lignin content (5.9%) was obtained with 6% H2O2 treatment for 4 h and 20 °C. This hemicellulose is much lighter in color than samples obtained at the midpoint condition and was found suitable for subsequent enzymatic hydrolysis. (AU)

• FAPESP's process: 08/03204-8 - Isolation of polysacharides from sugar cane bagasse to obtain oligosacharides using enzymatic hydrolysis and gel permeation chromatography
• Grantee: Adriane Maria Ferreira Milagres
• Support Opportunities: Regular Research Grants
• FAPESP's process: 06/03564-9 - Extraction of hemicellulose from sugarcane bagasse to obtain pentoses and xylooligossacharides
• Grantee: Michel Brienzo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)