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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of spectrophotometric methods for protein determination in waste stabilization ponds sample

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Author(s):
Adriana Cristina Poli Miwa ; Patrícia Bortoletto de Falco [2] ; Maria do Carmo Calijuri [3]
Total Authors: 3
Document type: Journal article
Source: Engenharia Sanitária e Ambiental; v. 13, n. 2, p. 236-242, 2008-06-00.
Field of knowledge: Engineering - Sanitary Engineering
Abstract

This research had as main objective to compare five spectrophotometric methods for protein determination in samples proceeding from sanitary effluent of treatment plant. Intention was to define a methodology that is of fast and easy and reliable application for this type of sample. The stabilization ponds, as systems of biological treatment, have as main constituent proteins, carbohydrates and lipids, but also they present many interfering composites, for example, phenolic urea, detergents and composites, that can harm the quantification of such parameters. The analyzed methods had been Lowry, Biuret, Bradford and Acid bicinconinic. The method of Lowry revealed more adequate to the characteristics of the sample, with good reproducibility, specific reagent, moderate cost and absence of interfering substance. (AU)