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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

COI barcode versus morphological identification of Culex ( Culex ) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil

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Author(s):
Magdalena Laurito ; Tatiane MP de Oliveira ; Walter Ricardo Almiron ; Maria Anice Mureb Sallum
Total Authors: 4
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 108, p. 110-122, 2013.
Abstract

Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species. (AU)

FAPESP's process: 11/20397-7 - Morphological and molecular taxonomy and phylogeny of Nyssorhynchus subgenus of Anopheles (Diptera: Culicidae), with special emphasis on Anopheles darlingi from Mata Atlântica
Grantee:Maria Anice Mureb Sallum
Support type: Regular Research Grants