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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antidepressant- and anticompulsive-like effects of purinergic receptor blockade: Involvement of nitric oxide

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Author(s):
Pereira, Vitor S. [1] ; Casarotto, Plinio C. [1] ; Hiroaki-Sato, Vinicius A. [1] ; Sartim, Ariandra G. [2] ; Guimaraes, Francisco S. [3, 1] ; Joca, Samia R. L. [3, 2]
Total Authors: 6
Affiliation:
[1] Campus USP, Sch Med, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Campus USP, Sch Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ctr Interdisciplinary Res Appl Neurosci NAPNA, BR-05508 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: European Neuropsychopharmacology; v. 23, n. 12, p. 1769-1778, DEC 2013.
Web of Science Citations: 25
Abstract

Activation of purinergic receptors by ATP (P2R) modulates glutamate release and the activation of post-synaptic P2R is speculated to induce nitric oxide (NO) synthesis. Increased glutamatergic and nitrergic signaling have been involved in the neurobiology of stress-related psychiatric disorders such as anxiety and depression. Therefore, the aim of this study was to test the effects of two P2R antagonists (PPADS and iso-PPADS) in animals submitted to models predictive of antidepressant-, anxiolytic- and anticompulsive-like effects. Swiss mice receiving PPADS at 12.5 mg/kg showed reduced immobility time in the forced swimming test (FST) similarly to the prototype antidepressant imipramine (30 mg/kg). This dose was also able to decrease the number of buried marbles in the marble-burying test (MBT), an anticompulsive-like effect. However, no effect was observed in animals submitted to the elevated plus maze (EPM) and to the open field test. The systemic administration of iso-PPADS, a preferential P2XR antagonist, also reduced the immobility time in FST, which was associated to a decrease in NOx levels in the prefrontal cortex. In addition, P2X7 receptor was found co-immunoprecipitated with neuronal nitric oxide synthase (NOS1) in the prefrontal cortex. These results suggest that P2X7, possibly coupled to NOS1, could modulate behavioral responses associated to stress-related disorders and it could be a new target for the development of more effective treatments for affective disorders. (C) 2013 Elsevier B.V. and ECNP. All rights reserved. (AU)

FAPESP's process: 11/17281-7 - Involvement of epigenetic mechanisms induced by DNA methylation on stress-induced behavioral consequences and on the expression of genes involved in the neurobiology of depression
Grantee:Sâmia Regiane Lourenço Joca
Support type: Regular Research Grants
FAPESP's process: 07/03685-3 - Typical and atypical neurotransmitters in neuropsychiatric disorders
Grantee:Francisco Silveira Guimaraes
Support type: Research Projects - Thematic Grants