Characterization of regulatory T cell (Treg) function in patients infected with Leishmania braziliensis

Author(s)

Costa, Diego L. ^[1] ; Guimaraes, Luiz H. ^[2] ; Cardoso, Thiago M. ^[2] ; Queiroz, Adriano ^[2] ; Lago, Ednaldo ^[2] ; Roselino, Ana M. ^[3] ; Bacellar, Olivia ^[2] ; Carvalho, Edgar M. ^[2] ; Silva, Joao S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Biochem & Immunol, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Bahia, Serv Immunol, HUPES, Salvador, BA - Brazil

^[3] Univ Sao Paulo, Div Dermatol, Med Sch Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: HUMAN IMMUNOLOGY; v. 74, n. 12, p. 1491-1500, DEC 2013.
• Web of Science Citations: 11

Abstract

Th1 immune responses are crucial for eliminating Leishmania parasites. However, despite strong Th1 responses, cutaneous leishmaniasis (CL) patients infected with Leishmania braziliensis develop the disease, while milder Th1 responses are found in sub-clinical (SC) infections. Therefore, CL patients may experience impaired regulatory T cell (Treg) function, causing excessive Th1 responses and tissue damage. To address this hypothesis, we characterized the function of circulating Tregs in L. braziliensis infected CL patients and compared them to Tregs from uninfected controls (UC) and SC subjects. The frequency of circulating Tregs was similar in CL patients, UC and SC subjects. Moreover, CL patients Tregs suppressed lymphocyte proliferation and PBMC pro-inflammatory cytokine production more efficiently than UC Tregs, and also produced higher levels of IL-10 than UC and SC Tregs. Furthermore, PBMC and mononuclear cells from lesions of CL patients responded normally to Treg-induced suppression. Therefore, the lesion development in CL patients infected with L. braziliensis is not associated with impairment in Treg function or failure of cells to respond to immunomodulation. Rather, the increased Treg activation in CL patients may impair parasite elimination, resulting in establishment of chronic infection. Thus, immunological strategies that interfere with this response may improve leishmaniasis treatment. (C) 2013 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 08/05982-8 - Modulation of immune responses to Leishmania braziliensis by regulatory T cells
• Grantee: Diego Luís Costa
• Support Opportunities: Scholarships in Brazil - Doctorate