CD95 (FAS) and CD178 (FASL) induce the apoptosis of CD4+and CD8+cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania spp

Author(s)

Oliveira Silva, Kathlenn Liezbeth ^[1] ; Melo, Larissa Martins ^[1] ; Perosso, Juliana ^[1] ; Oliveira, Bruna Brito ^[1] ; Patto dos Santos, Paulo Sergio ^[1] ; Eugenio, Flavia de Rezende ^[1] ; Felix de Lima, Valeria Marcal ^[1]

Affiliation

^[1] Univ Estadual Paulista, Fac Med Vet, FMVA UNESP, Sch Vet Sci , Sao Paulo State Univ, Dept Clin Surg & Anim Reprod, Lab Cellular Immunol, BR-16050680 Sao Paulo - Brazil

• Document type: Journal article
• Source: Veterinary Parasitology; v. 197, n. 3-4, p. 470-476, NOV 8 2013.
• Web of Science Citations: 5

Abstract

Infected dogs are urban reservoirs of Leishmania chagasi, which is a causative agent of visceral leishmaniasis (VL). Dogs exhibit immune suppression during the course of this disease, and lymphocyte apoptosis is involved in this process. To investigate apoptosis and the expression levels of FAS-FAS-associated death domain protein (CD95 or APO-1), FASL-FAS ligand protein (CD178), and TRAIL-TNF-related apoptosis-inducing ligand (CD253) receptors in peripheral blood mononuclear cells and spleen leukocytes from 38 symptomatic dogs with moderate VL and 25 healthy dogs were evaluated by flow cytometry. The apoptosis rate of blood and splenic CD4+ and CD8+ cells was higher in infected dogs than in healthy dogs. The expression levels of FAS and FASL in blood and splenic CD4+ cells were lower in infected dogs than in healthy dogs. FAS expression in CD8+ cells was higher in infected dogs than in healthy dogs; in contrast, FASL expression was lower in infected dogs. The expression of the TRAIL receptor increased only in splenic CD8+ cells from infected dogs. The FAS and FAS-L blocking antibodies confirmed the importance of these receptors in apoptosis. Our results enhance the current understanding of the immune response in dogs infected with L chagasi, facilitating the future development of therapeutic interventions to reduce lymphocyte depletion. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/02321-3 - Role of Fas and TNF in the apoptosis of CD4 T lymphocyte and CD8 T lymphocyte in dogs with visceral leishmaniasis.
• Grantee: Kathlenn Liezbeth Oliveira da Silva
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 11/06214-7 - Role of Fas and TNF in the apoptosis of CD4 T lymphocyte and CD8 T lymphocyte in dogs with visceral leishmaniasis
• Grantee: Valéria Marçal Felix de Lima
• Support type: Regular Research Grants