Fucan effect on CHO cell proliferation and migration

Author(s)

Duarte Barreto Nobre, Leonardo Thiago ^{[1, 2]} ; Jacome Vidal, Arthur Anthunes ^[1] ; Almeida-Lima, Jailma ^[1] ; Oliveira, Ruth Medeiros ^[1] ; Paredes-Gamero, Edgar Jean ^[2] ; Medeiros, Valquiria Pereira ^[2] ; Trindade, Edvaldo Silva ^[3] ; Cavichiolo Franco, Celia Regina ^[3] ; Nader, Helena Bonciani ^[2] ; Oliveira Rocha, Hugo Alexandre ^[1]

Affiliation

^[1] Univ Fed Rio Grande do Norte, Dept Bioquim, BR-59072970 Natal, RN - Brazil

^[2] Univ Fed Sao Paulo, Depto Bioquim, Disciplina Biol Mol, Sao Paulo - Brazil

^[3] Univ Fed Parana, Dept Biol Celular, BR-80060000 Curitiba, PR - Brazil

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 98, n. 1, p. 224-232, OCT 15 2013.
• Web of Science Citations: 9

Abstract

Fucan is a term used to denominate sulfated L-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schroederi seaweed. This 21.5 kDa galactofucan inhibited CHO-Kl proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin alpha 5 beta 1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs. (C) 2013 Elsevier Ltd. All rights reserved. (AU)