Identification of upregulated genes in oral squamous cell carcinomas

Author(s)

Lessa, Roberta C. ^{[1, 2]} ; Campos, Antonio Hugo J. F. M. ^[3] ; de Freitas, Carlos Elias ^[4] ; da Silva, Felipe Rodrigues ^[5] ; Kowalski, Luiz Paulo ^[2] ; Carvalho, Andre Lopes ^{[2, 6]} ; Vettore, Andre Luiz ^{[1, 7]}

Affiliation

^[1] Ludwig Inst Canc Res, Sao Paulo Branch, Sao Paulo - Brazil

^[2] AC Camargo Canc Hosp, Dept Head & Neck Surg, Sao Paulo - Brazil

^[3] AC Camargo Canc Hosp, Dept Pathol, Sao Paulo - Brazil

^[4] Bahia Sch Med & Publ Hlth, Salvador, BA - Brazil

^[5] Embrapa Informat Agr, Campinas, SP - Brazil

^[6] Barretos Canc Hosp, Dept Head & Neck Surg, Barretos - Brazil

^[7] Fed Univ Sao Paulo UNIFESP, Dept Sci Biol, Sao Paulo - Brazil

• Document type: Journal article
• Source: HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK; v. 35, n. 10, p. 1475-1481, OCT 2013.
• Web of Science Citations: 19

Abstract

BackgroundOral cancer is the most common subset of head and neck squamous cell carcinomas (HNSCC). These tumors often have an aggressive clinical outcome hallmarked by a propensity for local invasion and regional nodal metastasis. Upregulated genes could be useful as markers for diagnosis, prognosis, and as new drug targets for these tumors. MethodsTo identify upregulated genes in oral squamous cell carcinomas (OSSCs), we examined the ORESTES public database and used a quantitative reverse transcription-polymerase chain reaction (qRT-PCR) approach to determine the expression level of selected genes in tumor samples. Results and ConclusionsThe ORESTES data mining analysis indicated 40 upregulated genes in HNSCC. Nine of these candidate genes were selected for further qRT-PCR validation and 3 of them (ALDOA, AHSA1, and POLQ) were frequently found upregulated in OSCC samples, which may indicate an association of these genes with the carcinogenesis process in this tumor site and they can constitute potential new targets for therapy. (c) 2012 Wiley Periodicals, Inc. Head Neck 35: 1475-1481, 2013 (AU)

• FAPESP's process: 05/02580-8 - Evaluation of methylation profile as prognostic marker in patients with head and neck tumors
• Grantee: Andre Luiz Vettore de Oliveira
• Support Opportunities: Regular Research Grants