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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differences in collagen distribution of healthy and regenerated periodontium. Histomorphometric study in dogs

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Author(s):
Souza, Sergio L. S. [1] ; Macedo, Guilherme O. [1] ; Silveira e Souza, Adriana M. M. [2] ; Taba, Jr., Mario [1] ; Novaes, Jr., Arthur B. [1] ; Oliver, Constance [2] ; Jamur, Maria C. [2] ; Correa, Vani M. A. [2]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodont, BR-05508 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Cell & Mol Biol & Pathogen Bioagents, BR-05508 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: HISTOLOGY AND HISTOPATHOLOGY; v. 28, n. 10, p. 1315-1324, OCT 2013.
Web of Science Citations: 2
Abstract

Previous studies have shown that there is a relationship between periodontal disease and the distribution of collagen fibers. This study evaluated the distribution of collagen types I and III in regenerated bone and periodontal ligament, comparing them to the tissues near the regenerated area and to the healthy periodontium. In the third (P3) and fourth (P4) mandibular premolars of 5 healthy mongrel dogs, bilaterally, buccal class 2 furcation lesions were surgically created and chronified for 3 weeks. After that, full flaps were elevated and expanded polytetrafluoroethylene (e-PTFE) membranes were adapted, sutured and recovered by the flaps. Two weeks after surgery, two membranes on the same side were removed and the other membranes were removed four weeks after surgery. The dogs were euthanized at 12 weeks following placement of the e-PTFE membranes. P3 and P4 teeth as well as the second premolars (healthy control teeth) and their periodontal tissues were removed and histologically processed for Collagen Quantification (COLQ). The amount of type III collagen was higher in native bone compared to the regenerated area. For periodontal ligament, COLQ for type I collagen showed statistically significant differences (Tukeys's Multiple Comparison, p<0.05) between the regenerated groups and the control group. These differences were not found for type III COLQ. There are significant differences in collagen distribution among the regenerated, native and control tissues. Membrane removal 2 or 4 weeks postoperatively did not influence the collagen composition. (AU)