Increasing the density of markers around a major QTL controlling resistance to angular leaf spot in common bean

Author(s)

Oblessuc, Paula Rodrigues ^{[1, 2]} ; Kupper Cardoso Perseguini, Juliana Morini ^{[1, 2]} ; Baroni, Renata Moro ^{[1, 2]} ; Chiorato, Alisson Fernando ^[3] ; Morais Carbonell, Sergio Augusto ^[3] ; Costa Mondego, Jorge Mauricio ^[1] ; Vidal, Ramon Oliveira ^[4] ; Aranha Camargo, Luis Eduardo ^[5] ; Benchimol-Reis, Luciana Lasry ^[1]

Affiliation

^[1] IAC, Ctr Recursos Genet Vegetais, BR-13001970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet & Evolucao & Bioagentes, BR-13083970 Campinas, SP - Brazil

^[3] IAC, Ctr Graos & Fibras, BR-13001970 Campinas, SP - Brazil

^[4] Univ Montreal, CHU St Justine Res Ctr, Montreal, PQ - Canada

^[5] Univ Sao Paulo, ESALQ, Dept Fitopatol & Nematol, BR-13418900 Sao Paulo - Brazil

• Document type: Journal article
• Source: THEORETICAL AND APPLIED GENETICS; v. 126, n. 10, p. 2451-2465, OCT 2013.
• Web of Science Citations: 15

Abstract

Angular leaf spot (ALS) causes major yield losses in the common bean (Phaseolus vulgaris L.), an important protein source in the human diet. This study describes the saturation around a major quantitative trait locus (QTL) region, ALS10.1, controlling resistance to ALS located on linkage group Pv10 and explores the genomic context of this region using available data from the P. vulgaris genome sequence. DArT-derived markers (STS-DArT) selected by bulk segregant analysis and SCAR and SSR markers were used to increase the resolution of the QTL, reducing the confidence interval of ALS10.1 from 13.4 to 3.0 cM. The position of the SSR ATA220 coincided with the maximum LOD score of the QTL. Moreover, a new QTL (ALS10.2(UC)) was identified at the end of the same linkage group. Sequence analysis using the P. vulgaris genome located ten SSRs and seven STS-DArT on chromosome 10 (Pv10). Coincident linkage and genome positions of five markers enabled the definition of a core region for ALS10.1 spanning 5.3 Mb. These markers are linked to putative genes related to disease resistance such as glycosyl transferase, ankyrin repeat-containing, phospholipase, and squamosa-promoter binding protein. Synteny analysis between ALS10.1 markers and the genome of soybean suggested a dynamic evolution of this locus in the common bean. The present study resulted in the identification of new candidate genes and markers closely linked to a major ALS disease resistance QTL, which can be used in marker-assisted selection, fine mapping and positional QTL cloning. (AU)

• FAPESP's process: 09/02411-2 - Fine mapping of angular leaf spot resistance in common bean (Phaseolus vulgaris L.)
• Grantee: Paula Rodrigues Oblessuc
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/51673-7 - Candidate gene identification for angular leaf spot in common bean (Phaseolus vulgaris L.)
• Grantee: Luciana Lasry Benchimol-Reis
• Support Opportunities: Regular Research Grants