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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synthetic phosphoethanolamine induces cell cycle arrest and apoptosis in human breast cancer MCF-7 cells through the mitochondrial pathway

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Author(s):
Ferreira, Adilson Kleber [1, 2] ; Meneguelo, Renato [3] ; Pereira, Alexandre [4] ; Filho, Otaviano Mendonca R. [5] ; Chierice, Gilberto Orivaldo [3] ; Maria, Durvanei Augusto [1, 2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Med, Sao Paulo - Brazil
[2] Butantan Inst, Biochem & Biophys Lab, Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Chem & Polymers Technol, Sao Carlos, SP - Brazil
[4] Butantan Inst, Genet Lab, Sao Paulo - Brazil
[5] Univ Uberaba, Belo Horizonte, MG - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BIOMEDICINE & PHARMACOTHERAPY; v. 67, n. 6, p. 481-487, JUL 2013.
Web of Science Citations: 23
Abstract

Phosphoethanolamine (Pho-s) is a compound involved in phospholipid turnover, acting as a substrate for many phospholipids of the cell membranes. In a recent study, we showed that Pho-s has antitumor effect in the several tumor cells. In this study we evaluated the antitumor activity of synthetic Pho-s on MCF-7 breast cancer cells. Here we demonstrate that Pho-s is cytotoxic to MCF-7 cells in a dose-dependent manner, while it is cytotoxic to MCF10 only at higher concentrations. In addition, Pho-s induces a disruption in mitochondrial membrane potential (Delta psi m). Furthermore, Pho-s induces mitochondria aggregates in the cytoplasm and DNA fragmentation of MCF-7 cells visualized by confocal microscopy. In agreement with the reduction on Delta psi m, we showed that Pho-s induces apoptosis followed by an increase in cytochrome c expression and capase-3-like activity in MCF-7 cells. Our results demonstrate that Pho-s induces a cell cycle arrest in the G1 phase through an inhibition of cyclin D1 and stimulates p53. An additional highlight of this study is the finding that Pho-s inhibits Bcl-2, inducing apoptosis through the mitochondrial pathway. Taken together, these results show that Pho-s is a promising compound in the fight against cancer. (C) 2013 Elsevier Masson SAS. All rights reserved. (AU)