Evaluation of antitumoral liposomal formulation DODAC with synthetic compound phosphoethanolamine in human breast tumor cells

• Grant number: 14/02344-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2014
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Durvanei Augusto Maria
• Grantee: Manuela Garcia Laveli da Silva
• Host Institution: Instituto Butantan. São Paulo , SP, Brazil

Abstract

Breast cancer is the second most deadly cancer for women worldwide. Currently, conventional approaches to the treatment of breast cancer include radiotherapy, chemotherapy and hormone therapy. The resistance of tumor cells to chemotherapy is a major obstacle to the clinical success of cancer therapy. Synthetic phosphoethanolamine is a phosphorylated molecule artificially, which makes it different from its current level of absorption by approximately 90%, with anti-inflammatory properties and apoptotic molecules. Our group has developed several experiments involving the Pho-S with the liposomal vesicles and the results are fully satisfactory as to the efficacy of liposomal formulation. In this project the aim is to evaluate the anti-proliferative and pro-apoptotic effects in vitro liposomal formulation DODAC with synthetic phosphoethanolamine in human breast tumor cell line, MCF-7 effects. The effects of phosphoethanolamine cytotoxicity in tumor cell lines will be evaluated for viability by the MTT colorimetric assay and detection of IC50% by free radical production (LPO), changes in cell death pathways by flow cytometry. The intraestruturais changes by scanning electron and confocal laser microscopy. Marking of lysosomal vesicles and mitochondrial electrical potential probe rhodamine-123. The arrangement of the cytoskeleton by staining with phalloidin and quantification of cells in the senescence ²-galactosidase staining. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Manuela Garcia Laveli da. Antitumor evaluation of synthetic phosphoethanolamine and liposomal formulation DODAC/synthetic phosphoethanolamine in human breast tumor cells. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.