Morphological changes and parasite load of the adrenal from dogs with visceral leishmaniasis

Author(s)

Claudia Momo ^[1] ; Nathália Alves de Souza Rocha ^[2] ; Pamela Rodrigues Reina Moreira ^[1] ; Danísio Prado Munari ^[4] ; Suely Regina Mogami Bomfim ^[4] ; Daniela Bernadete Rozza ^[4] ; Rosemeri de Oliveira Vasconcelos ^[1]

Affiliation

^[1] Univ Estadual Paulista, FCAV, Dept Patol Vet, BR-14884900 Jaboticabal, SP - Brazil

^[2] Universidade Estadual Paulista – UNESP. Faculdade de Medicina Veterinária de Araçatuba – FMVA. Produção e Saúde Animal - Brasil

^[3] Universidade Estadual Paulista – UNESP. Faculdade de Ciências Agrárias e Veterinárias – FCAV. Departamento de Ciências Exatas - Brasil

^[4] Univ Estadual Paulista, FMVA, Dept Apoio Prod & Saude Anim, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: Revista Brasileira de Parasitologia Veterinária; v. 23, n. 1, p. 30-35, 2014-03-00.

Abstract

The objective of this study was to analyze morphological changes and parasite loads in the adrenal gland from 45 dogs with visceral leishmaniasis (VL). The animals were from the Zoonosis Control Center of Araçatuba, state of São Paulo, which is an endemic region for the disease. These animals were euthanized due to positive diagnoses of VL. The dogs were classified into asymptomatic, oligosymptomatic and symptomatic groups. The parasite load was determined by immunohistochemistry, using VL-positive dog hyperimmune serum. Nine dogs showed an inflammatory infiltrate composed, predominantly, of plasma cells and macrophages. However, only eight dogs showed macrophages with amastigote forms of the parasite, immunolabeled in the cytoplasm. The medullary and reticular layers were the most affected areas, possibly due to a favorable microenvironment created by hormones in these regions. The density of parasites in the glandular tissue was not associated with clinical signs of VL (P > 0.05). However, the presence of the parasite was always associated with the presence of a granulomatous inflammatory infiltrate. This gland may not be an ideal place for the parasite's multiplication, but the presence of injuries to the glandular tissue could influence the dog's immune system, thus favoring the parasite's survival in the host's different organs. (AU)

• FAPESP's process: 09/15736-7 - Hepatic natural killer cells (PIT) action in liver of dogs with visceral leishmaniasis
• Grantee: Pamela Rodrigues Reina Moreira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/07815-4 - MIF, iNOS and MHC class II expression in macrophages of lymph nodes of the dogs with visceral leishmaniasis
• Grantee: Rosemeri de Oliveira Vasconcelos
• Support Opportunities: Regular Research Grants