Activation of platelet-activating factor receptor exacerbates renal inflammation and promotes fibrosis

Author(s):Show less -

Correa-Costa, Matheus ^[1] ; Andrade-Oliveira, Vinicius ^[1] ; Braga, Tarcio T. ^[1] ; Castoldi, Angela ^[1] ; Aguiar, Cristhiane F. ^[1] ; Origassa, Clarice S. T. ^[2] ; Rodas, Andrea C. D. ^[1] ; Hiyane, Meire I. ^[1] ; Malheiros, Denise M. A. C. ^[3] ; Rios, Francisco J. O. ^{[1, 4]} ; Jancar, Sonia ^[1] ; Camara, Niels O. S. ^{[2, 1]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci 4, Dept Immunol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Div Nephrol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Pathol, BR-05508900 Sao Paulo - Brazil

^[4] Univ Glasgow, Inst Cardiovasc & Med Sci, Glasgow Cardiovasc Res Ctr, British Heart Fdn, Glasgow, Lanark - Scotland

• Document type: Journal article
• Source: LABORATORY INVESTIGATION; v. 94, n. 4, p. 455-466, APR 2014.
• Web of Science Citations: 14

Abstract

Platelet-activating factor (PAF) is a lipid mediator with important pro-inflammatory effects, being synthesized by several cell types including kidney cells. Although there is evidence of its involvement in acute renal dysfunction, its role in progressive kidney injury is not completely known. In the present study, we investigated the role of PAF receptor (PAFR) in an experimental model of chronic renal disease. Wild-type (WT) and PAFR knockout (KO) mice underwent unilateral ureter obstruction (UUO), and at kill time, urine and kidney tissue was collected. PAFR KO animals compared with WT mice present: (a) less renal dysfunction, evaluated by urine protein/creatinine ratio; (b) less fibrosis evaluated by collagen deposition, type I collagen, Lysyl Oxidase-1 (LOX-1) and transforming growth factor beta (TGF-beta) gene expression, and higher expression of bone morphogenetic protein 7 (BMP-7) (3.3-fold lower TGF-beta/BMP-7 ratio); (c) downregulation of extracellular matrix (ECM) and adhesion molecule-related machinery genes; and (d) lower levels of pro-inflammatory cytokines. These indicate that PAFR engagement by PAF or PAF-like molecules generated during UUO potentiates renal dysfunction and fibrosis and might promote epithelial-to-mesenchymal transition (EMT). Also, early blockade of PAFR after UUO leads to a protective effect, with less fibrosis deposition. In conclusion, PAFR signaling contributes to a pro-inflammatory environment in the model of obstructive nephropathy, favoring the fibrotic process, which lately will generate renal dysfunction and progressive organ failure. (AU)

• FAPESP's process: 12/02270-2 - New cellular, molecular and immunological mechanisms involved in acute and chronic renal injury: the search for new therapeutical approaches
• Grantee: Niels Olsen Saraiva Câmara
• Support Opportunities: Research Projects - Thematic Grants