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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Semen analysis of Golden Retriever healthy dogs and those affected by muscular dystrophy

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Author(s):
Peres, M. A. [1, 2] ; da Rocha, A. M. [3] ; Vannucchi, C. I. [2] ; Mendes, C. M. [2] ; Cavalcanti, P. V. [2] ; Nichi, M. [2] ; Ambrosio, C. E. [4] ; Miglino, M. A. [1] ; Visintin, J. A. [2] ; D'Avila Assumpcao, M. E. O. [2]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil
[3] Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI - USA
[4] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Basic Sci, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: ANDROLOGIA; v. 46, n. 3, p. 277-282, APR 2014.
Web of Science Citations: 3
Abstract

This study aimed to characterise canine flow cytometry semen analysis, as well as seminal reactive oxygen species dosage using the Golden Retriever breed as model of study. Moreover, we searched for the influence of muscular dystrophy in Golden Retriever dogs on semen parameters. Thirty-seven semen samples were obtained from healthy Golden Retrievers (n=15) and from muscular dystrophy affected dogs (n=22). Sperm-rich fractions were analysed by standardised breeding soundness examination in addition to the assay of fluorescence assisted cell sorting for acrosome integrity, mitochondrial activity and DNA fragmentation. Volume of ejaculate, per cent of motile spermatozoa and vigour were similar between groups; there were no differences in the per cent of minor and major defects. Integrity of acrosomal membrane, mitochondrial potential and sperm DNA fragmentation had no significant differences between groups either. Animals from control group had higher concentration of spontaneous seminal oxidative species in comparison with affected animals. Dogs affected by dystrophy had seminal parameters similar to those observed in healthy dogs except for the lower concentration of oxidative species. Future studies aiming to establish reference values for canine seminal parameters should be considered preferably with distinction of breeds. (AU)