Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups

Author(s)

Mennitti, Lais Vales ^[1] ; Oyama, Lila Missae ^[2] ; de Oliveira, Juliana Lopez ^[2] ; Losinskas Hachul, Ana Claudia ^[2] ; Santamarina, Aline Boveto ^[2] ; de Santana, Aline Alves ^[2] ; Okuda, Marcos Hiromu ^[2] ; Ribeiro, Eliane Beraldi ^[2] ; da Penha Oller do Nascimento, Claudia Maria ^[2] ; Pisani, Luciana Pellegrini ^[3]

Affiliation

^[1] Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Fisiol Nutr, Dept Fisiol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP - Brazil

• Document type: Journal article
• Source: LIPIDS IN HEALTH AND DISEASE; v. 13, FEB 5 2014.
• Web of Science Citations: 8

Abstract

Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring. Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean +/- standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant. Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group. Conclusion: In conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups. (AU)

• FAPESP's process: 11/19426-2 - Effect of diet enriched with prebiotics in the intracellular mechanism involved with development of insulin resistance in offspring, 21 and 90 days of life, of mothers treated with trans fatty acids, during pregnancy and lactation
• Grantee: Luciana Pellegrini Pisani
• Support Opportunities: Regular Research Grants