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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biological activity of nine recombinant AtRALF peptides: Implications for their perception and function in Arabidopsis

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Author(s):
do Canto, Amanda Morato [1] ; Ceciliato, Paulo H. O. [1] ; Ribeiro, Bianca [1] ; Ortiz Morea, Fausto Andres [1] ; Franco Garcia, Antonio Augusto [2] ; Silva-Filho, Marcio C. [3] ; Moura, Daniel S. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Lab Bioquim Prot, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Genet Estat, BR-13418900 Piracicaba, SP - Brazil
[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Lab Biol Mol Plantas, BR-13418900 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Plant Physiology and Biochemistry; v. 75, p. 45-54, FEB 2014.
Web of Science Citations: 29
Abstract

RALF is a small (5 kDa) and ubiquitous plant peptide signal. It was first isolated from tobacco leaf protein extracts owing to its capacity to alkalinize the extracellular media of cell suspensions. RALFs inhibit root growth and hypocotyl elongation, and a role for RALFs in cell expansion has also been proposed. Arabidopsis has 37 RALF isoforms (AtRALF), but only a small group of nine has high primary structure identity to the original RALF peptide isolated from tobacco. Herein, we report the heterologous production of these nine peptides in Escherichia coli and the evaluation of their activity in five biological assays. All AtRALF peptides produced showed strong alkalinizing activities, with the exception of the pollen-specific isoform AtRALF4. Although it exhibited no inhibitory activity in the root growth and hypocotyl elongation assays, AtRALF4 is a strong inhibitor of pollen germination. Our data demonstrate that the divergence in the tissue specificity and gene expression patterns of the different AtRALFs does not change the fact that their main role seems to be the regulation of cell expansion. Furthermore, different activities in the alkalinization assays upon the addition of two consecutive and saturating doses of the peptides suggest that the peptides are likely being sensed by specific receptors. (C) 2013 Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 08/52067-3 - Herbivory and intracellular transport of proteins
Grantee:Márcio de Castro Silva Filho
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants