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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Physical Damage on Giant Vesicles Membrane as a Result of Methylene Blue Photoirradiation

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Author(s):
Mertins, Omar [1] ; Bacellar, Isabel O. L. [2] ; Thalmann, Fabrice [3] ; Marques, Carlos M. [3] ; Baptista, Mauricio S. [2] ; Itri, Rosangela [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Fis, Dept Fis Aplicada, BR-01498 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil
[3] Univ Strasbourg, Inst Charles Sadron, CNRS, Strasbourg - France
Total Affiliations: 3
Document type: Journal article
Source: BIOPHYSICAL JOURNAL; v. 106, n. 1, p. 162-171, JAN 7 2014.
Web of Science Citations: 28
Abstract

In this study we pursue a closer analysis of the photodamage promoted on giant unilamellar vesicles membranes made of dioleoyl-sn-glycero-3-phosphocholine (DOPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), by irradiating methylene blue present in the giant unilamellar vesicles solution. By means of optical microscopy and electro-deformation experiments, the physical damage on the vesicle membrane was followed and the phospholipids oxidation was evaluated in terms of changes in the membrane surface area and permeability. As expected, oxidation modifies structural characteristics of the phospholipids that lead to remarkable membrane alterations. By comparing DOPC- with POPC-made membranes, we observed that the rate of pore formation and vesicle degradation as a function of methylene blue concentration follows a diffusion law in the case of DOPC and a linear variation in the case of POPC. We attributed this scenario to the nucleation process of oxidized species following a diffusion-limited growth regime for DOPC and in the case of POPC a homogeneous nucleation process. On the basis of these premises, we constructed models based on reaction-diffusion equations that fit well with the experimental data. This information shows that the outcome of the photosensitization reactions is critically dependent on the type of lipid present in the membrane. (AU)

FAPESP's process: 10/18163-5 - Membrane photo-protection as investigated by optical microscopy on giant unilamellar vesicles.
Grantee:Omar Mertins
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/50680-5 - Photosensitization in life sciences
Grantee:Mauricio da Silva Baptista
Support Opportunities: Research Projects - Thematic Grants