Aberrant Telomerase Expression in the Endometrium of Infertile Women with Deep Endometriosis

Author(s)

Mafra, Fenian A. ^[1] ; Christofolini, Denise M. ^[1] ; Cavalcanti, Viviane ^[1] ; Vilarino, Fabia L. ^[1] ; Andre, Gustavo M. ^[1] ; Kato, Patricia ^[2] ; Bianco, Bianca ^[1] ; Barbosa, Caio P. ^[1]

Affiliation

^[1] Fac Med ABC, Human Reprod & Genet Ctr, Ideia Feria Inst, BR-09060650 Santo Andre, SP - Brazil

^[2] Appl Biosyst Inc, Tech Support, Sao Paulo - Brazil

• Document type: Journal article
• Source: ARCHIVES OF MEDICAL RESEARCH; v. 45, n. 1, p. 31-35, JAN 2014.
• Web of Science Citations: 9

Abstract

Background and Aims. Considering the complex cellular and molecular mechanisms involved in endometriosis formation and progression and the similarities concerning the association of endometriosis with tumorigenesis and metastasis, we hypothesized a possible relationship between telomerase and the development/progression of endometriosis. The present study aimed to evaluate the expression of telomerase in the endometrium and peritoneal endometriotic lesions from women with endometriosis and controls. Methods. A case-control study was performed comprising 25 infertile women with endometriosis and 44 fertile women without endometriosis as controls. Samples of endometrium and endometriotic peritoneal lesions of the same patient were harvested in the late luteal phase of the cycle. The expression of hTERT and GAPDH genes was measured by mRNA using qRT-PCR based on TaqMan methodology. Student t test was used to compare the values between the groups; p > 0.05 was accepted as statistically significant. Results. The mean expression of hTERT in the endometriosis group was significantly high when compared to the control group (1.24 +/- 4.67 vs. 0.31 +/- 1.10, p = 0.026). When the expression of hTERT was compared in relation to disease stage, the group of moderate/severe endometriosis showed increased expression in relation to control group (2.59 +/- 7.35 vs. 0.31 +/- 1.10, p = 0.026). Regarding endometriotic peritoneal lesions, only one 1/25 expressed hTERT mRNA. This patient had deep endometriosis. Conclusions. There was an association between the expression of telomerase (hTERT mRNA) and the genesis and progression of endometriosis. (C) 2014 IMSS. Published by Elsevier Inc. (AU)

• FAPESP's process: 09/11298-5 - TELOMERASE EXPRESSION IN ENDOMETRIOSIS
• Grantee: Fernanda Abani Mafra
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/01431-7 - Study of Telomerase Expressio by qRT-PCR in Women With Peritoneal Endometriosis
• Grantee: Caio Parente Barbosa
• Support Opportunities: Regular Research Grants