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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Induction of immune response in broiler chickens immunized with recombinant FliC and challenged by Salmonella Typhimurium

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Author(s):
Baptista, Ana Angelita S. [1] ; Donato, Tais C. [1] ; Souza, Edmarcia E. [2] ; Goncalves, Guilherme A. M. [1] ; Garcia, Keila C. O. D. [1] ; Rodrigues, Joao C. Z. [1] ; Sequeira, Julio L. [1] ; Andreatti Filho, Raphael L. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista, UNESP, Fac Med Vet & Zootecnia, Dept Clin Vet, BR-18608970 Botucatu, SP - Brazil
[2] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 33, n. 10, p. 1215-1221, OCT 2013.
Web of Science Citations: 0
Abstract

The study examined (1) the immune response in broiler chickens after oral immunization with recombinant flagellin (rFliC) from Salmonella Typhimurium conjugated with sodium alginate microparticles, and the immune response enhancement in association with recombinant cholera toxin B subunit protein (rCTB) and pool of Lactobacillus spp. (PL). The immune responses were evaluated by dosage of IgY serum and IgA from intestinal fluid and immunostaining of CD8+ T lymphocytes in the cecum. The immunized animals were challenged with Salmonella Typhimurium (ST) 21 days after treatment. In all immunized groups, a significant increase (p<0.05) was observed in IgA levels (μg/mL), especially three weeks after immunization. The serum IgY levels (μg/mL) were little affected by the treatments and differed significantly among groups only in the second post-immunization week (p<0.05). After the challenge, the number of CD8+ T cells differed significantly between the treatments and negative control. Retrieval of Salmonella Typhimurium was not detected at 48 hours after the challenge in T2 (rFliC+rCTb), T3 (rFliC+PL) and T4 (rFliC+rCTB PL). The rFliC administered orally with or without rCTB and Lactobacillus spp. produces significant induction of humoral immune response, and the immunized chickens were more effective in eliminating Salmonella after challenge. (AU)

FAPESP's process: 09/53570-3 - Evaluation of immunostimulatory effect of lacvobacillusspp. expressing ctxB ltb, in poultry challenged with Salmonella enteritidis
Grantee:Raphael Lucio Andreatti Filho
Support Opportunities: Regular Research Grants