Simple-sequence repeat markers of Cattleya coccinea (Orchidaceae), an endangered species of the Brazilian Atlantic Forest

Author(s)

Novello, M. ^[1] ; Rodrigues, J. F. ^[1] ; Pinheiro, F. ^[2] ; Oliveira, G. C. X. ^[1] ; Veasey, E. A. ^[1] ; Koehler, S. ^[3]

Affiliation

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Piracicaba, SP - Brazil

^[2] Inst Bot Sao Paulo, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema, SP - Brazil

• Document type: Journal article
• Source: Genetics and Molecular Research; v. 12, n. 3, p. 3274-3278, 2013.
• Web of Science Citations: 6

Abstract

Microsatellite markers were developed for the endangered Brazilian orchid species Cattleya coccinea to describe its genetic diversity and structure and to support conservation studies. Nine microsatellite loci were isolated and characterized using an enriched genomic library. All loci are polymorphic at least in the 2 populations sampled, except for loci Cac05 and Cac09 for the Petropolis population. The mean number of alleles per locus was 8.8 between populations. The mean values of the observed and expected heterozygosities were 0.541 (ranging from 0 to 1) and 0.639 (ranging from 0 to 0.9), respectively. Cross-amplifications were performed in 7 additional Epidendroideae species, and at least 2 loci were successful in 3 additional Cattleya species, Epidendrum secundum, and Brasiliorchis gracilis. All markers described herein will be useful in further studies evaluating the genetic diversity, population dynamics, and conservation genetics of C. coccinea and related species. (AU)

• FAPESP's process: 11/18532-3 - Philogeography, population genetics and species delimitation of the complex Cattleya coccinea (Orchidaceae)
• Grantee: Elizabeth Ann Veasey
• Support Opportunities: Regular Research Grants