Effect of astaxanthin and fish oil on enzymatic antioxidant system and α-amylase activity of salivary glands from rats

Author(s)

Mariana Ferreira Leite ^[1] ; Amanda Martins de Lima ^[2] ; Simone Jee Sun Kang ^[3] ; Maria Teresa Botti Rodrigues dos Santos ^[4] ; Rosemari Otton ^[5]

Affiliation

^[1] Universidade Cruzeiro do Sul. Department of Pediatric Dentistry - Brasil

^[2] Universidade Cruzeiro do Sul. Department of Pediatric Dentistry - Brasil

^[3] Universidade Cruzeiro do Sul. Department of Pediatric Dentistry - Brasil

^[4] Universidade Cruzeiro do Sul. Department of Pediatric Dentistry - Brasil

^[5] Universidade Cruzeiro do Sul. Department of Health Sciences - Brasil

• Document type: Journal article
• Source: Braz. J. Oral Sci.; v. 13, n. 1, p. 58-63, 2014-03-00.

Abstract

Salivary glands contribute to oral health. It is therefore of interest to study therapies that may favor their function and protection. AIM: To evaluate the effect of astaxanthin, fish oil and association of them on enzymatic antioxidant system and functional parameters of salivary glands. METHODS: Healthy rats (n=32) were divided into 4 groups: untreated-control, astaxanthin-treated (1 mg/kg body weight- BW), fish oil-treated (10 mg EPA/kg BW and 7 mg DHA/kg BW), and fish oil plus astaxanthin-treated. A prophylactic dose was administered in each group daily by gavage, for 45 days. Superoxide dismutase (SOD), catalase, glutathione peroxidase, reductase, and α-amylase activities were determined in salivary glands and compared by ANOVA and Tukey post-test (p<0.05). RESULTS: Parotid gland presented increased catalase and glutathione system and unaffected SOD activity after astaxanthin and astaxanthin plus fish oil treatment (p<0.05). Fish oil stimulated only glutathione peroxidase activity of parotid gland (p<0.05). Submandibular gland presented stimulated SOD and catalase, and reduced glutathione reductase activities after fish oil and fish oil plus astaxanthin treatment (p<0.05). SOD and glutathione reductase activities were reduced by astaxanthin treatment in submandibular gland (p<0.05). Parotid gland presented increased α-amylase activity in all groups supplemented and submandibular glands presented no changes (p<0.05). CONCLUSIONS: Astaxanthin, fish oil and combination of them stimulated the antioxidant system and functional parameter of salivary glands, which could be beneficial to oral health. (AU)

• FAPESP's process: 09/12342-8 - The carotenoid astaxanthin as a redox switch in muscle tissues of rats submitted to exhaustive swimming exercise: the AstaSwim project
• Grantee: Andréa Ramalho Mihaliuc
• Support Opportunities: Scholarships in Brazil - Master