Electrochemical determination of estradiol using a thin film containing reduced graphene oxide and dihexadecylphosphate

Author(s)

Janegitz, Bruno C. ^[1] ; dos Santos, Fabricio A. ^[1] ; Faria, Ronaldo C. ^[2] ; Zucolotto, Valtencir ^[1]

Affiliation

^[1] Univ Sao Paulo, Nanomed & Nanotoxicol Grp, Inst Fis Sao Carlos, BR-13566390 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Quim, BR-13565970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 37, p. 14-19, APR 1 2014.
• Web of Science Citations: 33

Abstract

Graphene is a material that has attracted attention with regard to sensing and biosensing applications in recent years. Here, we report a novel treatment (using ultrasonic bath and ultrasonic tip) to obtain graphene oxide (GO) and a new stable conducting film using reduced graphene oxide (RGO) and dihexadecylphosphate film (DHP). The GO was obtained by chemical exfoliation and it was reduced using NaBH4. Subsequently, RGO-DHP dispersion was prepared and it was dropped onto a glassy carbon electrode by casting technique. The electrode was characterized by cyclic voltammetry and electrochemical spectroscopy impedance. The voltammetric behavior of the RGO-DHP/GC electrode in the presence of estradiol was studied, and the results reported an irreversible oxidation peak current at 0.6 V. Under the optimal experimental conditions, using linear sweep adsorptive stripping voltammetry, the detection limit obtained for this hormone was 7.7 x 10(-8) mol L-1. The proposed electrode can be attractive for applications as electrochemical sensors and biosensors. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/02877-4 - Development of New Biosensors Using Active Nanoesctrutured Materials
• Grantee: Bruno Campos Janegitz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral