Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair

Author(s)

Antonio Luiz Martins Maia Filho ^{[1, 2]} ; Fabrício Pires de Moura do Amaral ^[2] ; Airton Abrahão Martin ^[3] ; Luís Eduardo Silva Soares ^[4]

Affiliation

^[1] Fac Diferencial, Lab Fisiol, Teresina, PI - Brazil

^[2] Fac Santo Agostinho, Teresina, PI - Brazil

^[3] Faculdade Diferencial. Laboratório de Anatomia - Brasil

^[4] Universidade do Vale do Paraíba. Faculdade de Ciências da Saúde. Instituto de Pesquisa e Desenvolvimento - Brasil

^[5] Universidade do Vale do Paraíba. Faculdade de Ciências da Saúde. Instituto de Pesquisa e Desenvolvimento - Brasil

• Document type: Journal article
• Source: Brazilian Archives of Biology and Technology; v. 57, n. 3, p. 334-339, 2014-01-31.

Abstract

The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C - control, surgery procedure without cerami c implant, Group Ce - uncoated Al 2O3 implants (n = 6) and Group CeHA - apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm-1 and 2940 cm-1 evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test. Inorganic and organic content were higher in CeHA samples than in Ce and C (CeHA > Ce > C). FT-Raman spectroscopy showed that the higher the deposition of the organic matrix, more mineralization occurred. The micronucleus test showed that the uncoated and apatite-coated Al2O3 implants were non-cytotoxic and safe to in vivo applications. (AU)