Construction of biomimetic units from platelet-rich plasma associated with chaotic fluid using static mixers: prospects for new biotechnological processes for bone tissue regeneration.

• Grant number: 23/14547-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2024
• End date: February 28, 2026
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Willian Fernando Zambuzzi
• Grantee: Júlia Ferreira de Moraes
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Bone tissue is a specialized connective tissue which provides support for the body, so when it is injured or fractured there is a need for adequate bone healing and repair, without the formation of intermediate calluses. In this sense, the use of scaffolds is intended to boost this tissue recovery or replacement more efficiently with the use of materials composed of cells expanded in vitro with the intention of being bioresorbable while new tissue is formed. Among these types of support, polymeric materials stand out, with sodium alginate (SA) and methacrylated gelatin (GelMA) hydrogels as representatives, which complement each other to form a biocompatible environment, favorable to cells because they have functions and components similar to those of the extracellular matrix, biodegradable and porous, Thus, this project proposes the use of these polymeric biomaterials to develop a bone scaffold, together with the addition of lyophilized platelet-rich plasma (PRP) to its composition, which is a natural autologous biomaterial that contains sources of growth factors and glycoproteins. In order to mimic bone tissue, the scaffolds will be supplemented with ²-tricalcium phosphate, a bioceramic that contains components of the inorganic part of bone and whose characteristics include bioactivity and osteoconductivity. In order to standardize bioprinting and improve the homogenization of the media solution, the Kenics static mixer (KSM) will be used, in which it is possible to control its speed and printed patterns/layers, thus portraying the formation, composition and printing of a biotint with the prospect of application in bone treatments.