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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phylogenetic relationships of Leishmania species based on trypanosomatid barcode (SSU rDNA) and gGAPDH genes: Taxonomic revision of Leishmania (L.) infantum chagasi in South America

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Author(s):
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Marcili, Arlei [1] ; Speranca, Marcia Ap. [2] ; da Costa, Andrea P. [1] ; Madeira, Maria de F. [3] ; Soares, Herbert S. [1] ; Sanches, Camila de O. C. C. [2] ; Acosta, Igor da C. L. [1] ; Girotto, Aline [1] ; Minervino, Antonio H. H. [1] ; Horta, Mauricio C. [4] ; Shaw, Jeffrey J. [5] ; Gennari, Solange M. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet, Dept Med Vet Prevent & Saude Anim, BR-05508 Sao Paulo - Brazil
[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[3] Fundacao Oswaldo Cruz, Inst Pesquisa Clin Evandro Chagas, Rio De Janeiro, RJ - Brazil
[4] Univ Fed Vale Sao Franscisco, Colegiado Med Vet, Petrolina, PE - Brazil
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: INFECTION GENETICS AND EVOLUTION; v. 25, p. 44-51, JUL 2014.
Web of Science Citations: 23
Abstract

Phylogenetic studies on trypanosomatid barcode using V7V8 SSU rRNA and gGAPDH gene sequences have provided support for redefining some trypanosomatid species and positioning new isolates. The genus Leishmania is a slow evolving monophyletic group and including important human pathogens. The phylogenetic relationships of this genus have been determined by the natural history of its vertebrate hosts, vector specificity, clinical manifestations, geographical distribution and molecular approaches using different markers. Thus, in an attempt to better understand the phylogenetic relationships of Leishmania species, we performed phylogenetic analysis on trypanosomatid barcode using V7V8 SSU rRNA and gGAPDH gene sequences among a large number of Leishmania species and also several Brazilian visceral Leishmania infantum chagasi isolates obtained from dogs and humans. Our phylogenetic analysis strongly suggested that Leishmania hertigi and Leishmania equatoriensis should be taxonomically revised so as to include them in the genus Endotrypanum; and supported ancient divergence of Leishmania enriettii. This, together with recent data in the literature, throws light on the discussion about the evolutionary southern supercontinent hypothesis for the origin of Leishmania ssp. and validates L. infantum chagasi from Brazil, thus clearly differentiating it from L. infantum, for the first time. (C) 2014 The Authors. Published by Elsevier B.V. (AU)

FAPESP's process: 11/15311-6 - Diversity of parasites of the genus Leishmania and Trypanosoma in wild mammals of one conservation units and domestic animals from their surroundings in the State of Espírito Santo.
Grantee:Igor da Cunha Lima Acosta
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/19853-8 - Epidemiological study and ecological aspects of canine visceral leishmaniasis in Maranhão State.
Grantee:Andréa Pereira da Costa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/50886-7 - Isolation and morphological, biological and molecular characterization and multigenes phylogeography from species causing visceral leishmaniasis
Grantee:Arlei Marcili
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/20221-9 - Molecular and serological diagnostic of Leishmania species in clinical samples of patients and dogs from the Midwest of São Paulo, Brazil, presenting visceral leishmaniasis
Grantee:Marcia Aparecida Speranca
Support type: Regular Research Grants