Fluorescence Spectroscopy and Confocal Microscopy of the Mycotoxin Citrinin in Condensed Phase and Hydrogel Films

Author(s)

Lauer, Milena H. ^[1] ; Gehlen, Marcelo H. ^[1] ; de Jesus, Karen ^[1] ; Berlinck, Roberto G. S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Journal of Fluorescence; v. 24, n. 3, p. 745-750, MAY 2014.
• Web of Science Citations: 3

Abstract

The emission spectra, quantum yields and fluorescence lifetimes of citrinin in organic solvents and hydrogel films have been determined. Citrinin shows complex fluorescence decays due to the presence of two tautomers in solution and interconversion from excited-state double proton transfer (ESDPT) process. The fluorescence decay times associated with the two tautomers have values near 1 and 5 ns depending on the medium. In hydrogel films of agarose and alginate, fluorescence imaging showed that citrinin is not homogeneously dispersed and highly emissive micrometer spots may be formed. Fluorescence spectrum and decay analysis are used to recognize the presence of citrinin in hydrogel films using confocal fluorescence microscopy and spectroscopy. (AU)

• FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 11/18215-8 - Advanced fluorescence microscopy: nano and micro analysis of chemical and biological systems
• Grantee: Marcelo Henrique Gehlen
• Support Opportunities: Regular Research Grants