Isolation and characterization of cellulolytic bacteria from the Stain house Lake, Antarctica

Author(s)

Melo, Itamar S. ^[1] ; Zucchi, Tiago D. ^[1] ; Silva, Rafael E. ^[1] ; Vilela, Elke S. D. ^[1] ; Saber, Mirian Lobo ^[1] ; Rosa, Luiz H. ^[2] ; Pellizari, Vivian H. ^[3]

Affiliation

^[1] Embrapa Environm, BR-13820000 Sao Paulo - Brazil

^[2] Univ Fed Minas Gerais, Inst Biol Sci, Belo Horizonte, MG - Brazil

^[3] Univ Sao Paulo, Oceanog Inst, Sao Paulo - Brazil

• Document type: Journal article
• Source: Folia Microbiologica; v. 59, n. 4, p. 303-306, JUL 2014.
• Web of Science Citations: 2

Abstract

The main aim was to evaluate the occurrence of cellulolytic bacteria from the Stain house Lake, located at Admiralty Bay, Antarctica. Thick cotton string served as a cellulose bait for the isolation of bacteria. A total of 52 bacterial isolates were recovered and tested for their cellulase activity, and two of them, isolates CMAA 1184 and CMAA 1185, showed significant cellulolytic activity on carboxymethylcellulose agar plates. Phylogenetic analysis placed the isolates into the Bacillus 16S ribosomal RNA gene subclade. Both isolates produced a cold-active cellulase which may play a crucial role in this extreme environment. (AU)

• FAPESP's process: 11/14333-6 - Diversity and polyphasic taxonomy of actinobacteria isolated from reforestation areas
• Grantee: Tiago Domingues Zucchi
• Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers