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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High-Resolution Inflorescence Phenotyping Using a Novel Image-Analysis Pipeline, PANorama

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Crowell, Samuel [1] ; Falcao, Alexandre X. [2, 3] ; Shah, Ankur [2] ; Wilson, Zachary [2] ; Greenberg, Anthony J. [2] ; McCouch, Susan R. [1, 2]
Total Authors: 6
[1] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 - USA
[2] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 - USA
[3] Univ Estadual Campinas, Inst Comp, Dept Informat Syst, BR-13083852 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Plant Physiology; v. 165, n. 2, p. 479-495, JUN 2014.
Web of Science Citations: 22

Variation in inflorescence development is an important target of selection for numerous crop species, including many members of the Poaceae (grasses). In Asian rice (Oryza sativa), inflorescence (panicle) architecture is correlated with yield and grain-quality traits. However, many rice breeders continue to use composite phenotypes in selection pipelines, because measuring complex, branched panicles requires a significant investment of resources. We developed an open-source phenotyping platform, PANorama, which measures multiple architectural and branching phenotypes from images simultaneously. PANorama automatically extracts skeletons from images, allows users to subdivide axes into individual internodes, and thresholds away structures, such as awns, that normally interfere with accurate panicle phenotyping. PANorama represents an improvement in both efficiency and accuracy over existing panicle imaging platforms, and flexible implementation makes PANorama capable of measuring a range of organs from other plant species. Using high-resolution phenotypes, a mapping population of recombinant inbred lines, and a dense single-nucleotide polymorphism data set, we identify, to our knowledge, the largest number of quantitative trait loci (QTLs) for panicle traits ever reported in a single study. Several areas of the genome show pleiotropic clusters of panicle QTLs, including a region near the rice Green Revolution gene SEMIDWARF1. We also confirm that multiple panicle phenotypes are distinctly different among a small collection of diverse rice varieties. Taken together, these results suggest that clusters of small-effect QTLs may be responsible for varietal or subpopulation-specific panicle traits, representing a significant opportunity for rice breeders selecting for yield performance across different genetic backgrounds. (AU)

FAPESP's process: 11/03110-6 - 3D image analysis of plant roots for genotype-phenotype associations
Grantee:Alexandre Xavier Falcão
Support type: Scholarships abroad - Research