Erythropoiesis-driven regulation of hepcidin in human red cell disorders is better reflected through concentrations of soluble transferrin receptor rather than growth differentiation factor 15

Author(s):Show less -

Fertrin, Kleber Yotsumoto ^[1] ; Lanaro, Carolina ^[1] ; Franco-Penteado, Carla Fernanda ^[1] ; de Albuquerque, Dulcineia Martins ^[1] ; Bandeira de Mello, Mariana Rezende ^[1] ; Pallis, Flavia Rubia ^[1] ; Cavalcanti Bezerra, Marcos Andre ^[2] ; Domingues Hatzlhofer, Betania Lucena ^[3] ; Olbina, Gordana ^[4] ; Olalla Saad, Sara Terezinha ^[1] ; Araujo, Aderson da Silva ^[3] ; Westerman, Mark ^[4] ; Costa, Fernando Ferreira ^[1]

Affiliation

^[1] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, INCTS, Campinas, SP - Brazil

^[2] Fed Univ Pernambuco UFPE, Ctr Biol Sci, Recife, PE - Brazil

^[3] HEMOPE Hematol & Hemotherapy Ctr Pernambuco, Recife, PE - Brazil

^[4] Intrins LifeSci LLC, La Jolla, CA - USA

• Document type: Journal article
• Source: American Journal of Hematology; v. 89, n. 4, p. 385-390, APR 2014.
• Web of Science Citations: 13

Abstract

Growth differentiation factor 15 (GDF-15) is a bone marrow-derived cytokine whose ability to suppress iron regulator hepcidin in vitro and increased concentrations found in patients with ineffective erythropoiesis (IE) suggest that hepcidin deficiency mediated by GDF-15 may be the pathophysiological explanation for nontransfusional iron overload. We aimed to compare GDF-15 production in anemic states with different types of erythropoietic dysfunction. Complete blood counts, biochemical markers of iron status, plasma hepcidin, GDF-15, and known hepcidin regulators {[}interleukin-6 and erythropoietin (EPO)] were measured in 87 patients with red cell disorders comprising IE and hemolytic states: thalassemia, sickle cell anemia, and cobalamin deficiency. Healthy volunteers were also evaluated for comparison. Neither overall increased EPO, nor variable GDF-15 concentrations correlated with circulating hepcidin concentrations (P = 0.265 and P = 0.872). Relative hepcidin deficiency was found in disorders presenting with concurrent elevation of GDF-15 and soluble transferrin receptor (sTfR), a biomarker of erythropoiesis, and sTfR had the strongest correlation with hepcidin (r(S) = -0.584, P < 0.0001). Our data show that high concentrations of GDF-15 in vivo are not necessarily associated with pathological hepcidin reduction, and hepcidin deficiency was only found when associated with sTfR overproduction. sTfR elevation may be a necessary common denominator of erythropoiesis-driven mechanisms to favor iron absorption in anemic states and appears a suitable target for investigative approaches to iron disorders. Am. J. Hematol. 89:385-390, 2014. (c) 2013 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 08/57441-0 - Clinical, cellular and molecular alterations in hemoglobinopathies and other hereditary hemolytic anemias
• Grantee: Fernando Ferreira Costa
• Support type: Research Projects - Thematic Grants