Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)

Author(s):Show less -

Silva, Duilio M. Z. de A. ^[1] ; Pansonato-Alves, Jose Carlos ^[1] ; Utsunomia, Ricardo ^[1] ; Araya-Jaime, Cristian ^[1] ; Ruiz-Ruano, Francisco J. ^[2] ; Daniel, Sandro Natal ^[3] ; Hashimoto, Diogo Teruo ^[4] ; Oliveira, Claudio ^[1] ; Camacho, Juan Pedro M. ^[2] ; Porto-Foresti, Fabio ^[3] ; Foresti, Fausto ^[1]

Affiliation

^[1] Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Sao Paulo - Brazil

^[2] Univ Granada, Dept Genet, Granada - Spain

^[3] Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias, Sao Paulo - Brazil

^[4] Univ Estadual Paulista, CAUNESP, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 9, n. 4 APR 15 2014.
• Web of Science Citations: 46

Abstract

Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism. (AU)

• FAPESP's process: 11/16825-3 - Molecular and cytogenetic structure of B-chromosomes in Astyanax paranae (Characiformes, Characidae)
• Grantee: Duílio Mazzoni Zerbinato de Andrade Silva
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 10/17009-2 - Biodiversity and phylogenetic relationships of the genera Astyanax, Hemigrammus, Hyphessobrycon and Moenkhausia (Characiformes: Characidae)
• Grantee: Claudio de Oliveira
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants