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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)

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Author(s):
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Silva, Duilio M. Z. de A. [1] ; Pansonato-Alves, Jose Carlos [1] ; Utsunomia, Ricardo [1] ; Araya-Jaime, Cristian [1] ; Ruiz-Ruano, Francisco J. [2] ; Daniel, Sandro Natal [3] ; Hashimoto, Diogo Teruo [4] ; Oliveira, Claudio [1] ; Camacho, Juan Pedro M. [2] ; Porto-Foresti, Fabio [3] ; Foresti, Fausto [1]
Total Authors: 11
Affiliation:
[1] Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Sao Paulo - Brazil
[2] Univ Granada, Dept Genet, Granada - Spain
[3] Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias, Sao Paulo - Brazil
[4] Univ Estadual Paulista, CAUNESP, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 9, n. 4 APR 15 2014.
Web of Science Citations: 46
Abstract

Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism. (AU)

FAPESP's process: 11/16825-3 - Molecular and Cytogenetic Structure of B-chromosomes in Astyanax paranae (CHARACIFORMES, CHARACIDAE)
Grantee:Duílio Mazzoni Zerbinato de Andrade Silva
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/17009-2 - Biodiversity and phylogenetic relationships of the genera Astyanax, Hemigrammus, Hyphessobrycon and Moenkhausia (Characiformes: Characidae)
Grantee:Claudio de Oliveira
Support type: BIOTA-FAPESP Program - Thematic Grants