| Full text | |
| Author(s): |
Caualcanti, I. M. G.
[1]
;
Ricomini Filho, A. P.
[1]
;
Lucena-Ferreira, S. C.
[1]
;
da Silva, W. J.
[1]
;
Paes Leme, A. F.
[2]
;
Senna, P. M.
[1]
;
Del Bel Cury, A. A.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Periodontol & Prosthodont, Piracicaba, SP - Brazil
[2] Brazilian Biosciences Natl Lab LNBio, Mass Spectrometry Lab, BR-10000 Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | ARCHIVES OF ORAL BIOLOGY; v. 59, n. 7, p. 695-703, JUL 2014. |
| Web of Science Citations: | 13 |
| Abstract | |
Objective: The aim of this study was to evaluate the composition of the salivary pellicle (SP) and multispecies biofilm developed on titanium nitrided by cold plasma. Methods: Titanium discs were allocated into a control group (Ti) and an experimental group (TiN - titanium-nitrided by cold plasma). The disc surface topography was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The chemical composition of the disc surface was determined by X-ray photoelectron spectroscopy (XPS). Stimulated, clarified, and filtered saliva was used to form pellicles on the discs. Proteome analysis of the adsorbed SP proteins was performed by liquid chromatography-mass spectrometry (LC-MS). The surface free energy (SFE) was evaluated before and after SP formation. A multispecies biofilm composed of Actinomyces naeslundii, Streptococcus oralis, Streptococcus mutans, Fusobacterium nucleatum, Veillonella dispar, and Candida albicans was developed on the SP-coated discs. Viable microorganism counts were determined. The biomass and average thickness of biofilms were analyzed by confocal laser-scanning microscopy (CLSM) with COMSTAT software. The biofilm organization was visualized by SEM. Results: The surface topography was similar in both groups. The SFE of the TiN group did not differ from that of the Ti group (p > 0.05), although the adsorption of pellicle proteins increased the SFE in both pellicle-coated groups (p < 0.001). Different proteins were identified on the Ti and TiN surfaces. The amount of biofilm was similar for both groups (p = 0.416), but the counts of F. nucleatum and S. oralis were higher in the TiN group (p < 0.001). Similar biofilms were characterized by the COMSTAT data, CLSM images, and SEM images. Conclusion: The titanium nitrided by cold plasma exhibited differences in SP composition and multispecies microbial biofilm population compared to the control titanium surface. (C) 2014 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 10/07894-9 - Evaluation of multi-species biofilms formed on different substrata and exposed to salivary concentrations of antimicrobial agents: microorganisms quantification and biofilm tridimensional morphology |
| Grantee: | Altair Antoninha Del Bel Cury |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/03242-0 - Development of a multispecie biofilm on surface modified titanium used in oral rehabilitation with implants |
| Grantee: | Indira Moraes Gomes Cavalcanti |
| Support Opportunities: | Scholarships in Brazil - Master |