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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Salivary pellicle composition and multispecies biofilm developed on titanium nitrided by cold plasma

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Author(s):
Caualcanti, I. M. G. [1] ; Ricomini Filho, A. P. [1] ; Lucena-Ferreira, S. C. [1] ; da Silva, W. J. [1] ; Paes Leme, A. F. [2] ; Senna, P. M. [1] ; Del Bel Cury, A. A. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Periodontol & Prosthodont, Piracicaba, SP - Brazil
[2] Brazilian Biosciences Natl Lab LNBio, Mass Spectrometry Lab, BR-10000 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 59, n. 7, p. 695-703, JUL 2014.
Web of Science Citations: 13
Abstract

Objective: The aim of this study was to evaluate the composition of the salivary pellicle (SP) and multispecies biofilm developed on titanium nitrided by cold plasma. Methods: Titanium discs were allocated into a control group (Ti) and an experimental group (TiN - titanium-nitrided by cold plasma). The disc surface topography was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The chemical composition of the disc surface was determined by X-ray photoelectron spectroscopy (XPS). Stimulated, clarified, and filtered saliva was used to form pellicles on the discs. Proteome analysis of the adsorbed SP proteins was performed by liquid chromatography-mass spectrometry (LC-MS). The surface free energy (SFE) was evaluated before and after SP formation. A multispecies biofilm composed of Actinomyces naeslundii, Streptococcus oralis, Streptococcus mutans, Fusobacterium nucleatum, Veillonella dispar, and Candida albicans was developed on the SP-coated discs. Viable microorganism counts were determined. The biomass and average thickness of biofilms were analyzed by confocal laser-scanning microscopy (CLSM) with COMSTAT software. The biofilm organization was visualized by SEM. Results: The surface topography was similar in both groups. The SFE of the TiN group did not differ from that of the Ti group (p > 0.05), although the adsorption of pellicle proteins increased the SFE in both pellicle-coated groups (p < 0.001). Different proteins were identified on the Ti and TiN surfaces. The amount of biofilm was similar for both groups (p = 0.416), but the counts of F. nucleatum and S. oralis were higher in the TiN group (p < 0.001). Similar biofilms were characterized by the COMSTAT data, CLSM images, and SEM images. Conclusion: The titanium nitrided by cold plasma exhibited differences in SP composition and multispecies microbial biofilm population compared to the control titanium surface. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/03242-0 - Development of a multispecie biofilm on surface modified titanium used in oral rehabilitation with implants
Grantee:Indira Moraes Gomes Cavalcanti
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/07894-9 - Evaluation of multi-species biofilms formed on different substrata and exposed to salivary concentrations of antimicrobial agents: microorganisms quantification and biofilm tridimensional morphology
Grantee:Altair Antoninha Del Bel Cury
Support type: Regular Research Grants