Development of a multispecie biofilm on surface modified titanium used in oral rehabilitation with implants

• Grant number: 11/03242-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2011
• End date: June 30, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Altair Antoninha Del Bel Cury
• Grantee: Indira Moraes Gomes Cavalcanti
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The aim of this study is to evaluate the development of a multispecie biofilm on titanium modified surface used in oral rehabilitation with implants. For this study, specimens will be divided in two groups according on the surface treatment received: titanium non-nitrided (TiN) (control) and nitrided (TiN+) (experimental). The surface roughness and the surface free energy (SFE) on the specimens will be determined before and after the formation of acquired pellicle and treatments. The pellicle protein profile formed on specimens will be identified by mass spectrometry before and after treatment with cold plasma. After specimens measurement of surface roughness, surface free energy and sterilization by gamma radiation, they will be packed into 24 plate and will be immersed in saliva for the formation of the acquired pellicle. After acquired pellicle formation, the saliva will be removed and a new universal fluid (FUM) with inoculums containing microorganisms to be studied: Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, Streptococcus oralis e Candida albicans will be introduced. Every 24h the environment will be exchanged until the biofilm formation in 72h. The viable cells will be expressed by UFC/mm2 and structural analysis will be verified by confocal laser scanning microscope. The amount of the specimens and the statistical analysis will be determinate after pilots studies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAUALCANTI, I. M. G.; RICOMINI FILHO, A. P.; LUCENA-FERREIRA, S. C.; DA SILVA, W. J.; PAES LEME, A. F.; SENNA, P. M.; DEL BEL CURY, A. A.. Salivary pellicle composition and multispecies biofilm developed on titanium nitrided by cold plasma. ARCHIVES OF ORAL BIOLOGY, v. 59, n. 7, p. 695-703, JUL 2014. (10/07894-9, 11/03242-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAVALCANTI, Indira Moraes Gomes. Influence of titanium nitride by cold-plasma on formation and development of multiespecies biofilms. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.