Luminescent threat: toxicity of light stick attractors used in pelagic fishery

Author(s):Show less -

de Oliveira, Tiago Franco ^[1] ; Medeiros da Silva, Amanda Lucila ^[1] ; de Moura, Rafaela Alves ^[1] ; Bagattini, Raquel ^[2] ; Falcao de Oliveira, Antonio Anax ^[1] ; Gennari de Medeiros, Marisa Helena ^[3] ; Di Mascio, Paolo ^[3] ; de Arruda Campos, Ivan Persio ^[4] ; Barretto, Fabiano Prado ^[5] ; Henriques Bechara, Etelvino Jose ^{[3, 6]} ; de Melo Loureiro, Ana Paula ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Inst Ciencias & Saude, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

^[4] Univ Estadual Paulista, Inst Ciencias Exatas & Tecnol, Sao Paulo - Brazil

^[5] Global Garbage, Hamburg - Germany

^[6] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Diadema, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 4, JUN 19 2014.
• Web of Science Citations: 6

Abstract

Light sticks (LS) are sources of chemiluminescence commonly used in pelagic fishery, where hundreds are discarded and reach the shores. Residents from fishing villages report an improper use of LS contents on the skin. Given the scarce information regarding LS toxicity, the effects of LS solutions in cell cultures were evaluated herein. Loss of viability, cell cycle changes and DNA fragmentation were observed in HepG2 cell line and skin fibroblasts. A non-cytotoxic LS concentration increased the occurrence of the mutagenic lesion 1,N-6-epsilon dAdo in HepG2 DNA by three-fold. Additionally, in vitro incubations of spent LS contents with DNA generated dGuo-LS adducts, whose structure elucidation revealed the presence of a reactive chlorinated product. In conclusion, the LS contents were found to be highly cyto- and genotoxic. Our data indicate an urgent need for LS waste management guidelines and for adequate information regarding toxic outcomes that may arise from human exposure. (AU)

• FAPESP's process: 06/56530-4 - Carbonilic and redox stress associated with alpha-aminoketones and endogenous beta-ketoacids: mechanisms and biomarkers.
• Grantee: Etelvino José Henriques Bechara
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/21636-8 - Transformation of human bronchial epithelial cells by benzo[a]pyrene: metabolic redox processes and DNA methylation / demethylation
• Grantee: Tiago Franco de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/08616-8 - Characterization of a new biotransformation pathway of bisphenol A and quantitation of DNA lesions in HL-60 and MCF-7 cells
• Grantee: Ana Paula de Melo Loureiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/08617-4 - Variables on glycemic control and pathogenesis of diabetic nephropathy: research on the phenomenon of metabolic memory
• Grantee: Antonio Anáx Falcão de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 09/54816-6 - Xenobiotic effects in cultured human cells and study of mechanisms of cytotoxic and genotoxic damage
• Grantee: Ana Paula de Melo Loureiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
• Grantee: Ohara Augusto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC