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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunohistochemical detection of receptors for estrogen and progesterone in endometrial glands and stroma during the pospartum anestrus in Nelore (Bos taurus indicus) cows

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Author(s):
R.S. Lima [1] ; I. Martin [2] ; W.C. Marques Filho [3] ; M.M.P. Rodrigues [4] ; R. Laufer-Amorim [5] ; J.C.P. Ferreira [6]
Total Authors: 6
Affiliation:
[1] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia
[2] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia
[3] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia
[4] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia
[5] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia
[6] Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia
Total Affiliations: 6
Document type: Journal article
Source: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 63, n. 4, p. 791-798, 2011-08-00.
Abstract

Twenty-four postpartum anestrous Nelore purebred cows were used in the study. Anestrous was determined based on the reproductive history which was confirmed in each cow based on plasma progesterone concentration and by transrectal ultrasonography. Endometrial biopsies were collected. The animals were separated into two groups according to maximum follicular diameter- Group 1: follicles <6mm (n=12) and Group 2: follicles >-6mm follicles (n=12). The immunohistochemistry technique was employed to evaluate the presence of estrogen and progesterone receptors in the uterine glandular epithelium and stroma. High counts of positive nuclei and high intensity of immunostain for estrogen and progesterone receptors in the glandular epithelium and stroma were observed in the two groups. However, the immunostain intensity of progesterone receptors in the glandular epithelium was higher in Group 2 compared to Group 1. When glandular epithelium and stroma were compared within each group, the relative number of estrogen receptors in the Group 1 was higher in the glandular epithelium compared to stroma and the immunostain intensity for the progesterone receptor in Group 2 was higher in the glandular epithelium compared to stroma. The results suggest that the mechanisms that control the expression of endomerial receptors in the anestrus are similar to the ones observed during the estrus cycle. (AU)

FAPESP's process: 07/03778-1 - Evaluation of the expression of estrogen and progesterone receptors in the endometrium of Nelore cows (Bos taurus indicus) during postpartum anestrous
Grantee:Rafaela Sanchez de Lima
Support Opportunities: Scholarships in Brazil - Scientific Initiation