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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Typing class I HLA-A gene using a nested PCR-RFLP procedure

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Author(s):
E.C. Castelli [1] ; D.S. Gil [2] ; L.C.S. Veiga [3] ; J.L.V. de Camargo [4]
Total Authors: 4
Affiliation:
[1] Universidade Estadual Paulista. Faculdade de Medicina. Departamento de Patologia - Brasil
[2] Universidade Estadual Paulista. Faculdade de Medicina. Departamento de Patologia - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética - Brasil
[4] Universidade Estadual Paulista. Faculdade de Medicina. Departamento de Patologia - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 38, n. 6, p. 837-842, 2005-06-00.
Abstract

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations. (AU)